-
Long name
Rabbit Antibody against Human, Mouse MERTK, Non-conjugated
-
Synonym name
MERTK Human, Mouse Antibody
-
Species reactivity
Human,Mouse
-
Tested applications recommended dilutions
ELISA,WB,IHC;Recommended dilution:WB:1:200-1000,IHC:1:20-1:200
-
Conjugation
Non-conjugated
-
Antibody isotype
IgG
-
UniProt ID
Q12866
-
Format
Liquid, diluted in buffer
-
Buffer composition
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Purity
>95%
-
Purification method
Antigen Affinity Purified Antibodies
-
Antibody category
Polyclonal Antibodies
-
Immunogen type
Recombinant human Tyrosine-protein kinase Mer protein (840-999AA)
-
Immunogen species
Human
-
Alias
Proto-oncogene c-Mer,Receptor tyrosine kinase MerTK,MERTK,MER
-
Background
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.
-
Clonality
Polyclonal antibody
-
Shipping and storage
MERTK Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
-
Properties
If you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus, Oryctolagus cuniculus
-
About
Rabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
French translation
anticorps
-
Gene target
-
Gene symbol
MERTK
-
Short name
Anti-MERTK
-
Technique
anti human, Antibody, Rabbit, Mouse, anti, antibody to, anti human antibodies, antibodies against human proteins, antibodies for, antibody Conjugates, mouses
-
Host
Rabbit, Rabbits
-
Species
Mouse, Humans, Mouses
-
Alternative name
MERTK Antibody
-
Alternative technique
antibodies, rabbit-anti, murine
-
Alternative to gene target
c-mer proto-oncogene tyrosine kinase, c-mer and MER and RP38, MERTK and IDBG-66030 and ENSG00000153208 and 10461, transferase activity, Extracellular, Mertk and IDBG-205458 and ENSMUSG00000014361 and 17289, MERTK and IDBG-628865 and ENSBTAG00000005828 and 504429
-
