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Long name
Rabbit Antibody against Human, Mouse HDAC3, Non-conjugated
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Synonym name
HDAC3 Human, Mouse Antibody
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Species reactivity
Human,Mouse
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Tested applications recommended dilutions
ELISA,WB,IHC,IF,ChIP;Recommended dilution:WB:1:1000-1:5000,IHC:1:20-1:200,IF:1:50-1:200
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Conjugation
Non-conjugated
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Antibody isotype
IgG
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UniProt ID
O15379
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Format
Liquid, diluted in buffer
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Buffer composition
Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
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Purity
>95%
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Purification method
Protein G purified
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Antibody category
Polyclonal Antibodies
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Immunogen type
Recombinant human Histone deacetylase 3 protein (1-428AA)
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Immunogen species
Human
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Alias
HD3,RPD3-2,SMAP45,HDAC3
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Background
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Participates in the BCL6 transcriptional repressor activity by deacetylating the H3 'Lys-27' (H3K27) on enhancer elements, antagonizing EP300 acetyltransferase activity and repressing proximal gene expression. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.
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Clonality
Polyclonal antibody
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Shipping and storage
HDAC3 Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
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Properties
If you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Oryctolagus cuniculus
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About
Rabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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French translation
anticorps
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Gene target
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Gene symbol
HDAC3
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Short name
Anti-HDAC3
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Technique
anti human, Antibody, Rabbit, Mouse, anti, antibody to, anti human antibodies, antibodies against human proteins, antibodies for, antibody Conjugates, mouses
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Host
Rabbit, Rabbits
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Species
Mouse, Humans, Mouses
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Alternative name
HDAC3 Antibody
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Alternative technique
antibodies, rabbit-anti, murine
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Alternative to gene target
histone deacetylase 3, HD3 and RPD3 and RPD3-2, HDAC3 and IDBG-50725 and ENSG00000171720 and 8841, NAD-dependent histone deacetylase activity (H3-K18 specific), nuclei, Hdac3 and IDBG-140814 and ENSMUSG00000024454 and 15183, HDAC3 and IDBG-646121 and ENSBTAG00000017360 and 404125
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