Rabbit Anti Human YWHAE Antibody-FITC

• Catalog number
  CSB-PA026287DC01HU_100ug
• Price
  Please ask
• Size
  100ug

• Long name
  Rabbit Antibody against Human YWHAE, FITC
• Synonym name
  YWHAE Human Antibody
• Species reactivity
  Human
• Tested applications recommended dilutions
  ELISA
• Conjugation
  FITC
• Antibody isotype
  IgG
• UniProt ID
  P62258
• Format
  Liquid, diluted in buffer
• Buffer composition
  Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity
  >95%
• Purification method
  Protein G purified
• Antibody category
  Polyclonal Antibodies
• Immunogen type
  Recombinant human 14-3-3 protein epsilon protein (1-255AA)
• Immunogen species
  Human
• Alias
  14-3-3E,YWHAE
• Background
  Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
• Clonality
  Polyclonal antibody
• Shipping and storage
  YWHAE Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
• Properties
  If you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Cusabio Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cusabio FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional conjugation
  Anti-FITC Antibody
• About
  Rabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Latin name
  Oryctolagus cuniculus
• French translation
  anticorps

• Gene target
  YWHAE  
• Gene symbol
  YWHAE
• Short name
  Anti-YWHAE
• Technique
  anti human, Antibody, Rabbit, anti, FITC, antibody to, anti human antibodies, antibodies against human proteins, antibodies for, Fluorescein
• Host
  Rabbit, Rabbits
• Label
  FITC
• Species
  Human, Humans
• Alternative name
  YWHAE Antibody,FITC conjugated
• Alternative technique
  antibodies, rabbit-anti, fluorescine
• Alternative to gene target
  tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide, 14-3-3E and HEL2 and KCIP-1 and MDCR and MDS, YWHAE and IDBG-14629 and ENSG00000108953 and 7531, phosphoprotein binding, Plasma membranes, Ywhae and IDBG-200597 and ENSMUSG00000020849 and 22627, YWHAE and IDBG-632682 and ENSBTAG00000005664 and 282125

Gene info

• Identity
  HGNC:12851
• Gene
  YWHAE
• Long gene name
  tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon
• Synonyms gene name
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide
• Synonyms
  • FLJ45465
• Synonyms name
  • 14-3-3 epsilon
• GenBank acession
  • U54778
• Locus
  17p13.3
• Discovery year
  1993-09-20
• Entrez gene record
  7531
• Pubmed identfication
  • 9371399
• RefSeq identity
  • NM_006761
• Classification
  • 14-3-3 phospho-serine/phospho-threonine binding proteins
• VEGA ID
  OTTHUMG00000134316

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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