-
Long name
Rabbit Antibody against Human USP28, Non-conjugated
-
Synonym name
USP28 Human Antibody
-
Species reactivity
Human
-
Tested applications recommended dilutions
ELISA,WB,IHC;Recommended dilution:WB:1:1000-1:5000,IHC:1:20-1:200
-
Conjugation
Non-conjugated
-
Antibody isotype
IgG
-
UniProt ID
Q96RU2
-
Format
Liquid, diluted in buffer
-
Buffer composition
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Purity
>95%
-
Purification method
Antigen Affinity Purified Antibodies
-
Antibody category
Polyclonal Antibodies
-
Immunogen type
Recombinant human Ubiquitin carboxyl-terminal hydrolase 28 protein (430-580AA)
-
Immunogen species
Human
-
Alias
Ubiquitin carboxyl-terminal hydrolase 28,Deubiquitinating enzyme 28,Ubiquitin thioesterase 28,Ubiquitin-specific-processing protease 28,USP28,KIAA1515
-
Background
Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus. Deubiquitinates ZNF304, hence preventing ZNF304 degradation by the proteasome and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) in a subset of colorectal cancers (CRC) cells (PubMed:24623306).
-
Clonality
Polyclonal antibody
-
Shipping and storage
USP28 Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
-
Short name of the antigen
Ubiquitin carboxyl-terminal hydrolase 28
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
-
Properties
If you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
About
Rabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin name
Oryctolagus cuniculus
-
French translation
anticorps
