Rabbit Anti Human SMURF1 Antibody-Biotin
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Catalog numberCSB-PA875720LD01HU_100ug
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PricePlease ask
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Size100ug
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Long nameRabbit Antibody against Human SMURF1, Biotin
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Synonym nameSMURF1 Human Antibody
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Species reactivityHuman
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Tested applications recommended dilutionsELISA
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ConjugationBiotin
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Antibody isotypeIgG
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UniProt IDQ9HCE7
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FormatLiquid, diluted in buffer
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Buffer compositionPreservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
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Purity>95%
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Purification methodProtein G purified
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Antibody categoryPolyclonal Antibodies
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Immunogen typeRecombinant human E3 ubiquitin-protein ligase SMURF1 protein (198-374AA)
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Immunogen speciesHuman
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AliasE3 ubiquitin-protein ligase SMURF1,SMAD ubiquitination regulatory factor 1,SMAD-specific E3 ubiquitin-protein ligase 1,SMURF1,KIAA1625
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BackgroundE3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Plays a role in dendrite formation by melanocytes (PubMed:23999003).
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ClonalityPolyclonal antibody
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Shipping and storageSMURF1 Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
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Short name of the antigenE3 ubiquitin-protein ligase SMURF1
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
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PropertiesIf you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional conjugationBiotinylated
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AboutRabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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French translationanticorps
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Gene target
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Gene symbolSMURF1
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Short nameAnti-SMURF1
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Techniqueanti human, Antibody, Rabbit, anti, antibody to, anti human antibodies, antibodies against human proteins, antibodies for
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HostRabbit, Rabbits
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LabelBiotin
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SpeciesHuman, Humans
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Alternative nameSMURF1 Antibody,Biotin conjugated
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Alternative techniqueantibodies, rabbit-anti
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Alternative to gene targetSMAD specific E3 ubiquitin protein ligase 1, SMURF1 and IDBG-28871 and ENSG00000198742 and 57154, R-SMAD binding, nuclei, Smurf1 and IDBG-208404 and ENSMUSG00000038780 and 75788, SMURF1 and IDBG-640859 and ENSBTAG00000007118 and
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Gene info
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Identity
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Gene
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Long gene nameSMAD specific E3 ubiquitin protein ligase 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-07-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- HECT domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data