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Long name
Rabbit Antibody against Human SMAD5, Biotin
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Synonym name
SMAD5 Human Antibody
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Species reactivity
Human
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Tested applications recommended dilutions
ELISA
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Conjugation
Biotin
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Antibody isotype
IgG
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UniProt ID
Q99717
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Format
Liquid, diluted in buffer
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Buffer composition
Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
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Purity
>95%
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Purification method
Protein G purified
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Antibody category
Polyclonal Antibodies
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Immunogen type
Recombinant human Mothers against decapentaplegic homolog 5 protein (1-209AA)
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Immunogen species
Human
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Alias
Mothers against decapentaplegic homolog 5MAD homolog 5Mothers against DPP homolog 5,JV5-1,SMAD family member 5,SMAD 5,Smad5,hSmad5,SMAD5,MADH5
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Background
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).
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Clonality
Polyclonal antibody
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Shipping and storage
SMAD5 Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
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Short name of the antigen
Mothers against decapentaplegic homolog 5
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
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Properties
If you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional conjugation
Biotinylated
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About
Rabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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French translation
anticorps
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Gene target
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Gene symbol
SMAD5-AS1, SMAD5
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Short name
Anti-SMAD5
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Technique
anti human, Antibody, Rabbit, anti, antibody to, anti human antibodies, antibodies against human proteins, antibodies for
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Host
Rabbit, Rabbits
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Label
Biotin
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Species
Human, Humans
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Alternative name
SMAD5 Antibody,Biotin conjugated
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Alternative technique
antibodies, rabbit-anti
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Alternative to gene target
SMAD family member 5, DWFC and JV5-1 and MADH5, SMAD5 and IDBG-45903 and ENSG00000113658 and 4090, transforming growth factor beta receptor, nuclei, Smad5 and IDBG-157537 and ENSMUSG00000021540 and 17129, BT.69069 and IDBG-645857 and ENSBTAG00000021772 and 768050
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