Rabbit Anti Human PAM Antibody-Biotin
-
Catalog numberCSB-PA017417LD01HU_50ug
-
PricePlease ask
-
Size50ug
-
-
Long nameRabbit Antibody against Human PAM, Biotin
-
Synonym namePAM Human Antibody
-
Species reactivityHuman
-
Tested applications recommended dilutionsELISA
-
ConjugationBiotin
-
Antibody isotypeIgG
-
UniProt IDP19021
-
FormatLiquid, diluted in buffer
-
Buffer compositionPreservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
-
Purity>95%
-
Purification methodProtein G purified
-
Antibody categoryPolyclonal Antibodies
-
Immunogen typeRecombinant Human Peptidyl-glycine alpha-amidating monooxygenase protein (338-497AA)
-
Immunogen speciesHuman
-
AliasPeptidyl-glycine alpha-amidating monooxygenase, PAM, Peptidylglycine alpha-hydroxylating monooxygenase, PHMPeptidyl-alpha-hydroxyglycine alpha-amidating lyase, Peptidylamidoglycolate lyase, PAL, PAM
-
BackgroundBifunctional enzyme that catalyzes 2 sequential steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl(2-hydroxyglycine) intermediate that is dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity.
-
ClonalityPolyclonal antibody
-
Shipping and storagePAM Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
-
Short name of the antigenPeptidyl-glycine alpha-amidating monooxygenase
-
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
-
PropertiesIf you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Additional conjugationBiotinylated
-
AboutRabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin nameOryctolagus cuniculus
-
French translationanticorps
-
Gene target
-
Gene symbolPAM, MYCBP2, F11R
-
Short nameAnti-PAM
-
Techniqueanti human, Antibody, Rabbit, anti, antibody to, anti human antibodies, antibodies against human proteins, antibodies for
-
HostRabbit, Rabbits
-
LabelBiotin
-
SpeciesHuman, Humans
-
Alternative namePAM Antibody,Biotin conjugated
-
Alternative techniqueantibodies, rabbit-anti
-
Gene info
-
Identity
-
Gene
-
Long gene namepeptidylglycine alpha-amidating monooxygenase
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year1992-06-08
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameMYC binding protein 2
-
Synonyms gene name
- MYC binding protein 2, E3 ubiquitin protein ligase
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year2004-04-01
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Ring finger proteins
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameF11 receptor
-
Synonyms gene
-
Synonyms gene name
- junctional adhesion molecule 1
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2001-04-10
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Ig-like cell adhesion molecule family
- V-set domain containing
- CD molecules
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data