Rabbit Anti Human MDC1 Antibody-Biotin
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Catalog numberCSB-PA623929LD01HU_50ug
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PricePlease ask
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Size50ug
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Long nameRabbit Antibody against Human MDC1, Biotin
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Synonym nameMDC1 Human Antibody
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Species reactivityHuman
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Tested applications recommended dilutionsELISA
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ConjugationBiotin
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Antibody isotypeIgG
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UniProt IDQ14676
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FormatLiquid, diluted in buffer
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Buffer compositionPreservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
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Purity>95%
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Purification methodProtein G purified
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Antibody categoryPolyclonal Antibodies
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Immunogen typeRecombinant Human Mediator of DNA damage checkpoint protein 1 protein (1892-2082AA)
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Immunogen speciesHuman
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AliasMediator of DNA damage checkpoint protein 1,Nuclear factor with BRCT domains 1,MDC1,KIAA0170,NFBD1
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BackgroundRequired for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1.
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ClonalityPolyclonal antibody
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Shipping and storageMDC1 Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
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Short name of the antigenMediator of DNA damage checkpoint protein 1
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
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PropertiesIf you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional conjugationBiotinylated
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AboutRabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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French translationanticorps
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Gene target
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Gene symbolMDC1-AS1, MDC1
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Short nameAnti-MDC1
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Techniqueanti human, Antibody, Rabbit, anti, antibody to, anti human antibodies, antibodies against human proteins, antibodies for
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HostRabbit, Rabbits
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LabelBiotin
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SpeciesHuman, Humans
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Alternative nameMDC1 Antibody,Biotin conjugated
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Alternative techniqueantibodies, rabbit-anti
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Alternative to gene targetmediator of DNA-damage checkpoint 1, NFBD1, MDC1 and IDBG-76700 and ENSG00000137337 and 9656, FHA domain binding, nuclei, Mdc1 and IDBG-179187 and ENSMUSG00000061607 and 240087, MDC1 and IDBG-634528 and ENSBTAG00000025526 and 511168
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Gene info
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Identity
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Gene
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Long gene nameMDC1 antisense RNA 1
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Synonyms gene name
- MDC1 antisense RNA 1 (non-protein coding)
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Synonyms
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Locus
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Discovery year2011-04-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Antisense RNAs
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemediator of DNA damage checkpoint 1
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Synonyms gene name
- mediator of DNA-damage checkpoint 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-05-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data