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Long name
Rabbit Antibody against Human MASP1, FITC
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Synonym name
MASP1 Human Antibody
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Species reactivity
Human
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Tested applications recommended dilutions
ELISA
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Conjugation
FITC
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Antibody isotype
IgG
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UniProt ID
P48740
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Format
Liquid, diluted in buffer
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Buffer composition
Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
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Purity
>95%
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Purification method
Protein G purified
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Antibody category
Polyclonal Antibodies
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Immunogen type
Recombinant human Mannan-binding lectin serine protease 1 protein (20-699AA)
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Immunogen species
Human
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Alias
Mannan-binding lectin serine protease 1 (EC:3.4.21.-),MASP1,CRARF,CRARF1,PRSS5,Complement factor MASP-3,Complement-activating component of Ra-reactive factor,Mannose-binding lectin-associated serine protease 1,MASP-1,Mannose-binding protein-associated serine protease,Ra-reactive factor serine protease p100,RaRF,Serine protease 5,Mannan-binding lectin serine protease 1 heavy chain,Mannan-binding lectin serine protease 1 light chain
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Background
Functions in the lectin pathway of complement, which performs a key role in innate immunity by recognizing pathogens through patterns of sugar moieties and neutralizing them. The lectin pathway is triggered upon binding of mannan-binding lectin (MBL) and ficolins to sugar moieties which leads to activation of the associated proteases MASP1 and MASP2. Functions as an endopeptidase and may activate MASP2 or C2 or directly activate C3 the key component of complement reaction. Isoform 2 may have an inhibitory effect on the activation of the lectin pathway of complement or may cleave IGFBP5.
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Clonality
Polyclonal antibody
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Shipping and storage
MASP1 Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
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Properties
If you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Cusabio Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cusabio FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional conjugation
Anti-FITC Antibody
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About
Rabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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French translation
anticorps
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Gene target
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Gene symbol
MASP1
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Short name
Anti-MASP1
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Technique
anti human, Antibody, Rabbit, anti, FITC, antibody to, anti human antibodies, antibodies against human proteins, antibodies for, Fluorescein
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Host
Rabbit, Rabbits
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Label
FITC
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Species
Human, Humans
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Alternative name
MASP1 Antibody,FITC conjugated
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Alternative technique
antibodies, rabbit-anti, fluorescine
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Alternative to gene target
mannan-binding lectin serine peptidase 1 (C4/C2 activating component of Ra-reactive factor), 3MC1 and CRARF and CRARF1 and MAP1 and MAp44 and MASP and MASP3 and PRSS5 and RaRF, MASP1 and IDBG-69257 and ENSG00000127241 and 102725441,5648, calcium-dependent protein binding, Extracellular, Masp1 and IDBG-149706 and ENSMUSG00000022887 and 17174, MASP1 and IDBG-634626 and ENSBTAG00000012467 and 522347
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