Rabbit Anti Human IL4 Antibody-FITC
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Catalog numberCSB-PA011659YC01HU_50ug
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Price:
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Size50ug
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Long nameRabbit Antibody against Human IL4, FITC
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Synonym nameIL4 Human Antibody
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Species reactivityHuman
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Tested applications recommended dilutionsELISA
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ConjugationFITC
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Antibody isotypeIgG
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UniProt IDP05112
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FormatLiquid, diluted in buffer
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Buffer compositionPreservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
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Purity>95%
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Purification methodProtein G purified
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Antibody categoryPolyclonal Antibodies
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Immunogen typeRecombinant Human Interleukin-4 protein (25-153AA)
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Immunogen speciesHuman
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AliasInterleukin-4,IL-4,B-cell stimulatory factor 1,BSF-1,BinetrakinLymphocyte stimulatory factor 1,Pitrakinra,IL4
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BackgroundParticipates in at least several B-cell activation processes as well as of other cell types (PubMed:3016727). It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages (By similarity).
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ClonalityPolyclonal antibody
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Shipping and storageIL4 Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
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Short name of the antigenInterleukin-4
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
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PropertiesIf you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Cusabio Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cusabio FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional conjugationAnti-FITC Antibody
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GeneInterleukin 4 (IL4) is a cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. It is used for dendritic and T cell therapy. Upon activation by IL-4, Th2 cells subsequently produce additional IL-4 in a positive feedback loop. The cell that initially produces IL-4, thus inducing Th0 differentiation, has not been identified, but recent studies suggest that basophils may be the effector cell. It is closely related and has functions similar to Interleukin 13. Recombinant, GMP rec. E. coli interleukin-4 for cell culture supplied by GENTAUR. Free samples on request.
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AboutRabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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French translationanticorps
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Gene target
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Gene symbolIL4
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Short nameAnti-IL4
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Techniqueanti human, Antibody, Rabbit, anti, FITC, antibody to, anti human antibodies, antibodies against human proteins, antibodies for, Fluorescein
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HostRabbit, Rabbits
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LabelFITC
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SpeciesHuman, Humans
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Alternative nameIL4 Antibody,FITC conjugated
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Alternative techniqueantibodies, rabbit-anti, fluorescine
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Alternative to gene targetinterleukin 4, BCGF-1 and BCGF1 and BSF-1 and BSF1 and IL-4, IL4 and IDBG-42701 and ENSG00000113520 and 3565, growth factor activity, Extracellular, Il4 and IDBG-172024 and ENSMUSG00000000869 and 16189, IL4 and IDBG-644671 and ENSBTAG00000015957 and 280824
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Gene info
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Identity
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Gene
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Long gene nameinterleukin 4
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1988-08-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Interleukins
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data