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Long name
Rabbit Antibody against Human CD3E, Biotin
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Synonym name
CD3E Human Antibody
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Species reactivity
Human
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Tested applications recommended dilutions
ELISA
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Conjugation
Biotin
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Antibody isotype
IgG
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UniProt ID
P07766
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Format
Liquid, diluted in buffer
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Buffer composition
Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
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Purity
>95%
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Purification method
Protein G purified
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Antibody category
Polyclonal Antibodies
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Immunogen type
Recombinant Human T-cell surface glycoprotein CD3 epsilon chain protein (23-126AA)
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Immunogen species
Human
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Alias
T-cell surface glycoprotein CD3 epsilon chain,T-cell surface antigen T3/Leu-4 epsilon chain,CD3e,CD3E,T3E
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Background
The CD3 complex mediates signal transduction, resulting in T-cell activation and proliferation. Required for normal immune responses (PubMed:15546002, PubMed:8490660).
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Clonality
Polyclonal antibody
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Shipping and storage
CD3E Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
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Short name of the antigen
T-cell surface glycoprotein CD3 epsilon chain
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
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Properties
If you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional conjugation
Biotinylated
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About
Rabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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French translation
anticorps
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Gene target
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Gene symbol
CD3E
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Short name
Anti-CD3E
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Technique
anti human, Antibody, Rabbit, anti, antibody to, anti human antibodies, antibodies against human proteins, antibodies for
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Host
Rabbit, Rabbits
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Label
Biotin
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Species
Human, Humans
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Alternative name
CD3E Antibody,Biotin conjugated
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Alternative technique
antibodies, rabbit-anti
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Alternative to gene target
CD3e molecule, epsilon (CD3-TCR complex), IMD18 and T3E and TCRE, CD3E and IDBG-73617 and ENSG00000198851 and 916, protein heterodimerization activity, Cell surfaces, Cd3e and IDBG-159085 and ENSMUSG00000032093 and 12501, CD3E and IDBG-631260 and ENSBTAG00000015710 and 281054
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