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Long name
Rabbit Antibody against Human ATP5A1, Non-conjugated
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Synonym name
ATP5A1 Human Antibody
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Species reactivity
Human
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Tested applications recommended dilutions
ELISA,WB;Recommended dilution:WB:1:500-2000
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Conjugation
Non-conjugated
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Antibody isotype
IgG
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UniProt ID
P25705
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Format
Liquid, diluted in buffer
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Buffer composition
Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
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Purity
>95%
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Purification method
Protein G purified
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Antibody category
Polyclonal Antibodies
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Immunogen type
Recombinant human ATP synthase subunit alpha, mitochondrial protein (44-553AA)
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Immunogen species
Human
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Alias
ATP synthase subunit alpha, mitochondrial,ATP5A1,ATP5A, ATP5AL2, ATPM
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Background
Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites.
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Clonality
Polyclonal antibody
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Shipping and storage
ATP5A1 Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
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Properties
If you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Rabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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French translation
anticorps
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Gene target
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Gene symbol
ATP5F1A
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Short name
Anti-ATP5A1
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Technique
anti human, Antibody, Rabbit, anti, antibody to, anti human antibodies, antibodies against human proteins, antibodies for, antibody Conjugates
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Host
Rabbit, Rabbits
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Species
Human, Humans
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Alternative name
ATP5A1 Antibody
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Alternative technique
antibodies, rabbit-anti
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Alternative to gene target
ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle, ATP5A and ATP5AL2 and ATPM and hATP1 and HEL-S-123m and MC5DN4 and MOM2 and OMR and ORM, ATP5A1 and IDBG-2902 and ENSG00000152234 and 498, hydrolase activity, Plasma membranes, Atp5a1 and IDBG-160170 and ENSMUSG00000025428 and 11946, ATP5A1 and IDBG-644576 and ENSBTAG00000002507 and 282578
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