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Long name
Rabbit Antibody against Human ARHGEF7, FITC
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Synonym name
ARHGEF7 Human Antibody
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Species reactivity
Human
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Tested applications recommended dilutions
ELISA
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Conjugation
FITC
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Antibody isotype
IgG
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UniProt ID
Q14155
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Format
Liquid, diluted in buffer
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Buffer composition
Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
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Purity
>95%
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Purification method
Protein G purified
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Antibody category
Polyclonal Antibodies
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Immunogen type
Recombinant Human Rho guanine nucleotide exchange factor 7 protein (1-250AA)
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Immunogen species
Human
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Alias
Beta-Pix,COOL-1,PAK-interacting exchange factor beta,p85,ARHGEF7,COOL1, KIAA0142, P85SPR, PAK3BP, PIXB ORF Names: Nbla10314
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Background
Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. Functions in cell migration, attachment and cell spreading. Promotes targeting of RAC1 to focal adhesions By similarity. May function as a positive regulator of apoptosis. Downstream of NMDA receptors and CaMKK-CaMK1 signaling cascade, promotes the formation of spines and synapses in hippocampal neurons.
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Clonality
Polyclonal antibody
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Shipping and storage
ARHGEF7 Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
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Properties
If you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Cusabio Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cusabio FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional conjugation
Anti-FITC Antibody
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About
Rabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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French translation
anticorps
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Gene target
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Gene symbol
ARHGEF7-IT1, ARHGEF7-AS2, ARHGEF7-AS1, ARHGEF7
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Short name
Anti-ARHGEF7
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Technique
anti human, Antibody, Rabbit, anti, FITC, antibody to, anti human antibodies, antibodies against human proteins, antibodies for, Fluorescein
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Host
Rabbit, Rabbits
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Label
FITC
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Species
Human, Humans
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Alternative name
ARHGEF7 Antibody,FITC conjugated
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Alternative technique
antibodies, rabbit-anti, fluorescine
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Alternative to gene target
Rho guanine nucleotide exchange factor (GEF) 7, BETA-PIX and COOL-1 and COOL1 and Nbla10314 and P50 and P50BP and P85 and P85COOL1 and P85SPR and PAK3 and PIXB, ARHGEF7 and IDBG-51160 and ENSG00000102606 and 8874, protein kinase binding, Cell surfaces, Arhgef7 and IDBG-132947 and ENSMUSG00000031511 and 54126, ARHGEF7 and IDBG-633975 and ENSBTAG00000020726 and 524352
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