Rabbit Anti Human ACTN4 Antibody-Biotin

• Catalog number
  CSB-PA00819D0Rb_50ug
• Price
  Please ask
• Size
  50ug

• Long name
  Rabbit Antibody against Human ACTN4, Biotin
• Synonym name
  ACTN4 Human Antibody
• Species reactivity
  Human
• Tested applications recommended dilutions
  ELISA
• Conjugation
  Biotin
• Antibody isotype
  IgG
• UniProt ID
  O43707
• Format
  Liquid, diluted in buffer
• Buffer composition
  Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Purity
  >95%
• Purification method
  Protein G purified
• Antibody category
  Polyclonal Antibodies
• Immunogen type
  Recombinant Human Alpha-actinin-4 protein (6-277AA)
• Immunogen species
  Human
• Alias
  Alpha-actinin-4Curated,Non-muscle alpha-actinin 4,ACTN4
• Background
  F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (PubMed:15772161). Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions (By similarity). May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA (PubMed:22351778).
• Clonality
  Polyclonal antibody
• Shipping and storage
  ACTN4 Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
• Short name of the antigen
  Alpha-actinin-4
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
• Properties
  If you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional conjugation
  Biotinylated
• About
  Rabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Latin name
  Oryctolagus cuniculus
• French translation
  anticorps

• Gene target
  ACTN4  
• Gene symbol
  ACTN4
• Short name
  Anti-ACTN4
• Technique
  anti human, Antibody, Rabbit, anti, antibody to, anti human antibodies, antibodies against human proteins, antibodies for
• Host
  Rabbit, Rabbits
• Label
  Biotin
• Species
  Human, Humans
• Alternative name
  ACTN4 Antibody,Biotin conjugated
• Alternative technique
  antibodies, rabbit-anti
• Alternative to gene target
  actinin, alpha 4, ACTININ-4 and FSGS and FSGS1, ACTN4 and IDBG-49384 and ENSG00000130402 and 81, actin filament binding, nuclei, Actn4 and IDBG-165033 and ENSMUSG00000054808 and 60595, ACTN4 and IDBG-637203 and ENSBTAG00000011215 and 522269

Gene info

• Identity
  HGNC:166
• Gene
  ACTN4
• Long gene name
  actinin alpha 4
• Synonyms gene
  • FSGS1
• Synonyms gene name
  • focal segmental glomerulosclerosis 1
• GenBank acession
  • D89980
• Locus
  19q13.2
• Discovery year
  1992-03-26
• Entrez gene record
  81
• Pubmed identfication
  • 9461087
  • 10700177
• Classification
  • Actinins
• VEGA ID
  OTTHUMG00000137382

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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