Rabbit Anti FITC Antibody-Non-conjugated

• Catalog number
  CSB-PA00511I0Rb_50ug
• Price
  Please ask
• Size
  50ug

• Long name
  Rabbit Antibody against FITC, Non-conjugated
• Synonym name
  FITC Antibody
• Tested applications recommended dilutions
  ELISA
• Conjugation
  Non-conjugated
• Antibody isotype
  IgG
• Format
  Liquid, diluted in buffer
• Buffer composition
  Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity
  >95%
• Purification method
  Protein G purified
• Antibody category
  Polyclonal Antibodies
• Immunogen type
  Fluorescein Isothiocyanate (Native Protein)
• Alias
  Fluorescein Isothiocyanate antibody
• Background
  FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.
• Clonality
  Polyclonal antibody
• Shipping and storage
  FITC Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Properties
  If you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Cusabio Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cusabio FITC is highly sensitive to pH extremes.
• Additional conjugation
  Anti-FITC Antibody
• About
  Rabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Latin name
  Oryctolagus cuniculus
• French translation
  anticorps

• Gene target
  Non-conjugated  
• Short name
  Anti-FITC
• Technique
  Antibody, Rabbit, anti, FITC, antibody to, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
• Host
  Rabbit, Rabbits
• Label
  FITC
• Alternative name
  FITC Antibody
• Alternative technique
  antibodies, rabbit-anti, fluorescine

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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