Rabbit Anti Escherichia coli O157:H7 tir Antibody-Non-conjugated
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Long name
Rabbit Antibody against Escherichia coli O157:H7 tir, Non-conjugated
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Synonym name
tir Escherichia coli O157:H7 Antibody
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Species reactivity
Escherichia coli O157:H7
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Tested applications recommended dilutions
ELISA
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Conjugation
Non-conjugated
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Antibody isotype
IgG
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UniProt ID
C6UYL8
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Format
Liquid, diluted in buffer
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Buffer composition
Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
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Purity
>95%
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Purification method
Protein G purified
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Antibody category
Polyclonal Antibodies
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Immunogen type
Recombinant Escherichia coli O157:H7 Translocated intimin receptor Tir protein (252-362AA)
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Immunogen species
Escherichia coli O157:H7
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Alias
Translocated intimin receptor Tir,Secreted effector protein Tir,tir,espE,ECSP_4676
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Background
Multifunctional protein that is required for efficient pedestal formation in host epithelial cells during infection. The extracellular region acts as a receptor for bacterial intimin, allowing the bacterium to attach tightly to the host-cell surface. Simultaneously, the intracellular region initiates a signaling cascade in the host cell, which leads to actin polymerization and formation of actin pedestals at the sites of bacterial adhesion (By similarity).
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Clonality
Polyclonal antibody
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Shipping and storage
tir Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
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Short name of the antigen
Translocated intimin receptor Tir
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
If you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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About
Rabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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French translation
anticorps
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Gene target
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Short name
Anti-tir
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Technique
Antibody, Rabbit, anti, antibody to, antibodies against human proteins, antibodies for, antibody Conjugates
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Host
Rabbit, Escherichia coli, Rabbits
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Alternative name
tir Antibody
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Alternative technique
antibodies, rabbit-anti, escherichia
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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