Rabbit Anti Escherichia coli lexA Antibody-Non-conjugated

• Catalog number
  CSB-PA363985HA01ENV_100ug
• Price
  Please ask
• Size
  100ug

• Long name
  Rabbit Antibody against Escherichia coli lexA, Non-conjugated
• Synonym name
  lexA Escherichia coli Antibody
• Species reactivity
  Escherichia coli
• Tested applications recommended dilutions
  ELISA,WB;Recommended dilution:WB:1:500-1:5000
• Conjugation
  Non-conjugated
• Antibody isotype
  IgG
• UniProt ID
  P0A7C2
• Format
  Liquid, diluted in buffer
• Buffer composition
  Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Purity
  >95%
• Purification method
  Protein G purified
• Antibody category
  Polyclonal Antibodies
• Immunogen type
  Recombinant Escherichia coli LexA repressor protein (1-202AA)
• Immunogen species
  Escherichia coli
• Alias
  LexA repressor,lexA
• Background
  Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. Binds to the 16 bp palindromic sequence 5'-CTGTATATATATACAG-3'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair. Implicated in hydroxy radical-mediated cell death induced by hydroxyurea treatment (PubMed:20005847).The SOS response controls an apoptotic-like death (ALD) induced (in the absence of the mazE-mazF toxin-antitoxin module) in response to DNA damaging agents that is mediated by RecA and LexA (PubMed:22412352).
• Clonality
  Polyclonal antibody
• Shipping and storage
  lexA Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
• Short name of the antigen
  LexA repressor
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Properties
  If you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• About
  Rabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Latin name
  Oryctolagus cuniculus
• French translation
  anticorps

• Gene target
  Escherichia   coli   lexA   Non-conjugated  
• Short name
  Anti-lexA
• Technique
  Antibody, Rabbit, anti, antibody to, antibodies against human proteins, antibodies for, antibody Conjugates
• Host
  Rabbit, Escherichia coli, Rabbits
• Alternative name
  lexA Antibody
• Alternative technique
  antibodies, rabbit-anti, escherichia

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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