Rabbit Anti Arabidopsis thaliana SPT16 Antibody-Biotin
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Catalog numberCSB-PA022955LD01DOA_50ug
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PricePlease ask
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Size50ug
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Long nameRabbit Antibody against Arabidopsis thaliana SPT16, Biotin
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Synonym nameSPT16 Arabidopsis thaliana Antibody
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Species reactivityArabidopsis thaliana
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Tested applications recommended dilutionsELISA
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ConjugationBiotin
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Antibody isotypeIgG
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UniProt IDO82491
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FormatLiquid, diluted in buffer
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Buffer compositionPreservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
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Purity>95%
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Purification methodProtein G purified
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Antibody categoryPolyclonal Antibodies
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Immunogen typeRecombinant Arabidopsis thaliana FACT complex subunit SPT16 protein (890-1074AA)
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Immunogen speciesArabidopsis thaliana
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AliasFacilitates chromatin transcription complex subunit SPT16,SPT16,At4g10710,T12H20.3
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BackgroundComponent of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II (Probable).
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ClonalityPolyclonal antibody
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Shipping and storageSPT16 Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
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Short name of the antigenFACT complex subunit SPT16
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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PropertiesIf you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Additional conjugationBiotinylated
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AboutRabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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French translationanticorps
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Gene target
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Gene symbolSUPT16H
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Short nameAnti-SPT16
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TechniqueAntibody, Rabbit, anti, antibody to, antibodies against human proteins, antibodies for
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HostRabbit, Rabbits
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LabelBiotin
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Alternative nameSPT16 Antibody,Biotin conjugated
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Alternative techniqueantibodies, rabbit-anti
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Gene info
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Identity
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Gene
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Long gene nameSPT16 homolog, facilitates chromatin remodeling subunit
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Synonyms gene name
- suppressor of Ty (S.cerevisiae) 16 homolog
- suppressor of Ty 16 homolog (S. cerevisiae)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1996-08-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Armadillo like helical domain containing
- M24 metallopeptidase family
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data