Human Telomerase reverse transcriptase (TERT)

• Catalog number
  CSB-EP023391HU-500ug
• Price
  Please ask
• Size
  500ug

• Description
  Recombinant Human Telomerase reverse transcriptase(TERT),partial expressed in E.coli
• Specifications
  Uniprot ID: O14746; Host: Homo sapiens (Human); Expression system: E.coli; Expression region: 281-436aa; Sequence information: Partial; Tag: N-terminal 6xHis-tagged; Purity: Greater than 85% as determined by SDS-PAGE.
• Additional_information
  MW: 22.7 kDa; Buffer composition: Tris-based buffer with 50% glycerol.
• Storage_and_shipping
  Shipped on ice packs (+4°C). The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Repeated freezing and thawing should be avoided. Store working aliquots at 4°C for up to one week.
• Notes
  For research use only.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional description
  Reverse transcription primers are used in PCR but in vivo reverse transcription begins when the viral particle that enters the cytoplasm of a target cell with its reverse transcriptase. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized. The process of reverse transcription generates, in the cytoplasm, a linear DNA via an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA . Extant models for reverse transcription propose that two specialized template switches known as strand-transfer reactions or “jumps” are required to generate the LTRs.
• Goup
  reverse transcription

• Gene target
  Telomerase   transcriptase   TERT  
• Gene symbol
  TERT
• Short name
  Telomerase reverse transcriptase (TERT)
• Technique
  reverse
• Species
  Human, Humans
• Alternative name
  H. sapiens Telomerase reverse transcriptase (telomerase reverse transcriptase)
• Alternative technique
  transctription
• Alternative to gene target
  telomerase reverse transcriptase, CMM9 and DKCA2 and DKCB4 and EST2 and hEST2 and hTRT and PFBMFT1 and TCS1 and TP2 and TRT, TERT and IDBG-10998 and ENSG00000164362 and 7015, telomeric RNA binding, nuclei, Tert and IDBG-165912 and ENSMUSG00000021611 and 21752, BT.60929 and IDBG-632169 and ENSBTAG00000012567 and 518884

Gene info

• Identity
  HGNC:11730
• Gene
  TERT
• Long gene name
  telomerase reverse transcriptase
• Synonyms
  • TRT
  • TP2
  • TCS1
  • hEST2
  • EST2
• GenBank acession
  • AF015950
• Locus
  5p15.33
• Discovery year
  1998-01-21
• Pubmed identfication
  • 9252327
• VEGA ID
  OTTHUMG00000090357
• Locus Specific Databases
  • LRG_343

MeSH Data

• Name
  Reverse Transcriptase Polymerase Chain Reaction
• Concept
  Scope note: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
• Tree numbers
  • E05.393.620.500.725
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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