Human Prostate stem cell antigen (PSCA)

• Catalog number
  CSB-EP018840HUa0-10ug
• Price
  Please ask
• Size
  10ug

• Description
  Recombinant Human Prostate stem cell antigen(PSCA) expressed in E.coli
• Specifications
  Uniprot ID: O43653; Host: Homo sapiens (Human); Expression system: E.coli; Expression region: 21-95a; Sequence information: Full Length of Mature Protein; Tag: N-terminal 6xHis-tagged; Purity: Greater than 90% as determined by SDS-PAGE.
• Additional_information
  MW: 12.2 kDa; Buffer composition: Tris-based buffer with 50% glycerol.
• Storage_and_shipping
  Shipped on ice packs (+4°C). The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Repeated freezing and thawing should be avoided. Store working aliquots at 4°C for up to one week.
• Notes
  For research use only.
• Additional description
  Antigens are peptides or recombinant or native dependent on the production method. For cells, cell lines and tissues in culture till half confluency.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Test
  Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.

• Gene target
  Prostate   stem   PSCA  
• Gene symbol
  PSCA
• Short name
  Prostate stem antigen (PSCA)
• Technique
  antigen, antigenes
• Species
  Human, Humans
• Alternative name
  H. sapiens Prostate progenitor cellular protein (PSCA)
• Alternative technique
  antigenes
• Tissue
  cell, prostate, stem

Gene info

• Identity
  HGNC:9500
• Gene
  PSCA
• Long gene name
  prostate stem cell antigen
• Synonyms
  • lncPSCA
• GenBank acession
  • AF043498
• Locus
  8q24.3
• Discovery year
  1999-08-25
• Entrez gene record
  8000
• Pubmed identfication
  • 9465086
• RefSeq identity
  • NM_005672
• Classification
  • LY6/PLAUR domain containing
• VEGA ID
  OTTHUMG00000162077

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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