Human Embryonic stem cell-related gene protein (HESRG)
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Catalog number
CSB-EP632275HU-500ug
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Price
Please ask
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Size
500ug
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Description
Recombinant Human Embryonic stem cell-related gene protein(HESRG) expressed in E.coli
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Specifications
Uniprot ID: Q1W209; Host: Homo sapiens (Human); Expression system: E.coli; Expression region: 1-222aa; Sequence information: Full Length; Tag: N-terminal 6xHis-tagged; Purity: Greater than 90% as determined by SDS-PAGE.
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Additional_information
MW: 28.2 kDa; Buffer composition: Tris-based buffer with 50% glycerol.
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Storage_and_shipping
Shipped on ice packs (+4°C). The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Repeated freezing and thawing should be avoided. Store working aliquots at 4°C for up to one week.
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Notes
For research use only.
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Additional description
For cells, cell lines and tissues in culture till half confluency. cDNA genes are a locus (or region) of DNA for functional transcript RNA or protein. An ELISA is used to detect the expressed protein in biological fluids, serum, saliva.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Test
Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.
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