Human E3 ubiquitin-protein ligase TRAIP (TRAIP)

Description

Recombinant Human E3 ubiquitin-protein ligase TRAIP(TRAIP) expressed in E.coli
Specifications

Uniprot ID: Q9BWF2; Host: Homo sapiens (Human); Expression system: E.coli; Expression region: 1-469aa; Sequence information: Full Length; Tag: N-terminal 6xHis-tagged; Purity: Greater than 85% as determined by SDS-PAGE.
Additional_information

MW: 58.8 kDa; Buffer composition: Tris-based buffer with 50% glycerol.
Storage_and_shipping

Shipped on ice packs (+4°C). The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Repeated freezing and thawing should be avoided. Store working aliquots at 4°C for up to one week.
Notes

For research use only.
Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Gene target

ubiquitin-protein ligase TRAIP TRAIP
Gene symbol

TRAIP
Short name

E3 ubiquitin-protein ligase TRAIP (TRAIP)
Species

Human, Humans
Alternative name

H. sapiens E3 ubiquitin-protein ligase TRAF interacting protein (TRAF interacting protein)
Alternative to gene target

TRAF interacting protein, RNF206 and TRIP, TRAIP and IDBG-35609 and ENSG00000183763 and 10293, metal ion binding, nuclei, Traip and IDBG-198670 and ENSMUSG00000032586 and 22036, TRAIP and IDBG-645894 and ENSBTAG00000004877 and 529769

Name

Ligase Chain Reaction
Concept

Scope note: A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
Tree numbers
- E05.393.620.311
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data