Escherichia coli Lipoate-protein ligase A (lplA)

Description

Recombinant Escherichia coli Lipoate-protein ligase A(lplA) expressed in E.coli
Specifications

Uniprot ID: C4ZT68; Host: Escherichia coli (strain K12 / MC4100 / BW2952); Expression system: E.coli; Expression region: 1-338aa; Sequence information: Full Length; Tag: N-terminal 6xHis-SUMO-tagged; Purity: Greater than 90% as determined by SDS-PAGE.
Additional_information

MW: 53.9 kDa; Buffer composition: Tris-based buffer with 50% glycerol.
Storage_and_shipping

Shipped on ice packs (+4°C). The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Repeated freezing and thawing should be avoided. Store working aliquots at 4°C for up to one week.
Notes

For research use only.

Name

Ligase Chain Reaction
Concept

Scope note: A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
Tree numbers
- E05.393.620.311
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data