Escherichia coli DNA replication and repair protein RecF (recF)

  • Catalog number
    CSB-EP358973ENV-200ug
  • Price
    Please ask
  • Size
    200ug
  • Description
    Recombinant Escherichia coli DNA replication and repair protein RecF(recF) expressed in E.coli
  • Specifications
    Uniprot ID: P0A7H0; Host: Escherichia coli (strain K12); Expression system: E.coli; Expression region: 2-357aa; Sequence information: Full Length of Mature Protein; Tag: N-terminal 6xHis-SUMO-tagged; Purity: Greater than 90% as determined by SDS-PAGE.
  • Additional_information
    MW: 56.4 kDa; Buffer composition: Tris-based buffer with 50% glycerol.
  • Storage_and_shipping
    Shipped on ice packs (+4°C). The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Repeated freezing and thawing should be avoided. Store working aliquots at 4°C for up to one week.
  • Notes
    For research use only.
  • Test
    Replication factor subunits and DNA replication proteins are found in DNA replication complexes like GINS, MCM,.. and replication initiators producing two identical replicas of DNA from one original DNA molecule. This process occurs in all living organisms and is the basis for biological inheritance. DNA is made up of a double helix of two strands, and each strand of the original DNA molecule gene serves as a template for the production of the complementary strand, a process referred to as semiconservative replication. Cellular proofreading and error-checking mechanisms ensure near perfect fidelity for DNA replication of genes.
  • Gene target
    Escherichia   coli   DNA   repair   protein   RecF   recF  
  • Gene symbol
    PRKDC, HLA-DOA
  • Short name
    Escherichia coli DNA repair protein RecF (recF)
  • Technique
    dna
  • Host
    Escherichia coli
  • Alternative name
    Escherichia coli Desoxyribonucleic acid replication and repair protein RecF (recF)
  • Alternative technique
    escherichia
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: A technique which uses synthetic oligonucleotides to direct the cell's inherent DNA repair system to correct a mutation at a specific site in an episome or chromosome.
  • Tree numbers
    • E02.095.301.500
    • E05.393.420.301.500
    • E05.393.420.601.575.500
  • Qualifiers
    legislation & jurisprudence, ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
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