Vascular Cell Adhesion Molecule 1 (VCAM1) Monoclonal Antibody (Human), FITC

• Catalog number
  MAA547Hu22-1ml-FITC
• Price
  Please ask
• Size
  1ml

• Description
  A Mouse monoclonal antibody against Human Vascular Cell Adhesion Molecule 1 (VCAM1). This antibody is labeled with FITC.
• Specifications
  Host: Mouse; Species Reactivity: Human; Clonality: monoclonal; Tested applications: WB; IHC; ICC; IP.; Concentration: 1mg/mL; Isotype: IgG2b Kappa; Conjugation: FITC
• Additional_information
  Sequence of the immunogen: Gln109~Ser318(23); Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
• Storage_and_shipping
  Upon receipt, store at -20°C or -80°C. Prepare working aliqotes prior to storage to avoid repeated freeze-thaw cycles.
• Notes
  Research Use Only.
• Additional description
  cell adhesion molecules play a role in cell growth and activation and are often identified by WB or ELISA as in the Vascular Adhesion Molecule 1 (VCAM1) Monoclonal Antibody ( ), FITC. For cells, cell lines and tissues in culture till half confluency. Whole adhesion and interacting molecules are  present in lysates used as reference for ELISA quantification of these molecules and their subunits.
• Properties
  If you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Cloud Clone Corp Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cloud Clone Corp FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Conjugation
  Anti-FITC Antibody
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• French translation
  anticorps

• Gene target
  Vascular   Adhesion   Molecule   VCAM1   Monoclonal  
• Gene symbol
  VCAM1
• Short name
  Vascular Adhesion Molecule 1 (VCAM1) Monoclonal Antibody ( ), FITC
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies
• Label
  FITC
• Species
  Human, Humans
• Alternative name
  Vascular cellular Adhesion Molecule 1 (vascular cellular adhesion molecule 1) monoclonal (antibody to-) (H. sapiens), fluorecein
• Alternative technique
  antibodies, fluorescine
• Alternative to gene target
  vascular cell adhesion molecule 1, CD106 and INCAM-100, VCAM1 and IDBG-100525 and ENSG00000162692 and 7412, cell adhesion molecule binding, Extracellular, Vcam1 and IDBG-189855 and ENSMUSG00000027962 and 22329
• Tissue
  cell, vascular

Gene info

• Identity
  HGNC:12663
• Gene
  VCAM1
• Long gene name
  vascular cell adhesion molecule 1
• Synonyms
  • CD106
• GenBank acession
  • M60335
• Locus
  1p21.2
• Discovery year
  1991-07-10
• Entrez gene record
  7412
• RefSeq identity
  • NM_001078
• Classification
  • CD molecules
  • Ig-like cell adhesion molecule family
  • Receptor ligands
  • C2-set domain containing
  • I-set domain containing
• VEGA ID
  OTTHUMG00000010982

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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