Tumor Necrosis Factor Alpha (TNFa) Monoclonal Antibody (Pig), FITC
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Catalog number
MAA133Po21-200ul-FITC
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Price
Please ask
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Size
200ul
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Description
A Mouse monoclonal antibody against Pig Tumor Necrosis Factor Alpha (TNFa). This antibody is labeled with FITC.
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Specifications
Host: Mouse; Species Reactivity: Pig; Clonality: monoclonal; Tested applications: WB; Concentration: 1mg/mL; Isotype: IgG; Conjugation: FITC
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Additional_information
Sequence of the immunogen: Leu77~Leu232; Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
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Storage_and_shipping
Upon receipt, store at -20°C or -80°C. Prepare working aliqotes prior to storage to avoid repeated freeze-thaw cycles.
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Notes
Research Use Only.
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Additional description
The Tumor Necrosis Factor Alpha (TNFa) Monoclonal Antibody ( ), FITC is a α- or alpha protein sometimes glycoprotein present in blood. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
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Properties
If you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Cloud Clone Corp Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cloud Clone Corp FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Pigs and the smaller guinea pigs are frequent used as models for humans.
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French translation
anticorps
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Gene target
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Short name
Tumor Necrosis Factor Alpha (TNFa) Monoclonal Antibody ( ), FITC
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Technique
Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies
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Label
FITC
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Species
Pig, Pigs and Guinea pigs
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Alternative name
Tumor Necrosis Factor a (TNFa) monoclonal (antibody to-) (swine), fluorecein
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Alternative technique
antibodies, fluorescine
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Tissue
tumor
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MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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