Serum Response Factor (SRF) Polyclonal Antibody (Human), FITC
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Catalog number
PAA515Hu01-10ml-FITC
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Price
Please ask
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Size
10ml
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Description
A Rabbit polyclonal antibody against Human Serum Response Factor (SRF). This antibody is labeled with FITC.
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Specifications
Host: Rabbit; Species Reactivity: Human; Clonality: polyclonal; Tested applications: WB, IHC; Concentration: 500ug/ml; Isotype: IgG; Conjugation: FITC
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Additional_information
Sequence of the immunogen: SRF (Thr231~Gln474); Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
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Storage_and_shipping
Upon receipt, store at -20°C or -80°C. Prepare working aliqotes prior to storage to avoid repeated freeze-thaw cycles.
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Notes
Research Use Only.
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Properties
If you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Cloud Clone Corp Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cloud Clone Corp FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional description
Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells. Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
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Conjugation
Anti-FITC Antibody
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Group
Polyclonals and antibodies, Sera
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Serum should be stored at +5°C
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French translation
anticorps
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Gene target
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Gene symbol
SRF, SPATA6
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Short name
Serum Response Factor (SRF) Polyclonal Antibody ( ), FITC
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Technique
Polyclonal, Antibody, serum, FITC, antibodies against human proteins, antibodies for, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Label
FITC
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Species
Human, Humans
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Alternative name
blood serum Response Factor (serum response factor (c-fos serum response element-binding transcription factor)) polyclonal (antibody to-) (H. sapiens), fluorecein
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Alternative technique
polyclonals, antibodies, sera, fluorescine
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Alternative to gene target
serum response factor (c-fos serum response element-binding transcription factor), MCM1, SRF and IDBG-87849 and ENSG00000112658 and 6722, protein dimerization activity, nuclei, Srf and IDBG-187355 and ENSMUSG00000015605 and 20807, BT.51656 and IDBG-631571 and ENSBTAG00000012777 and 533039
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Tissue
serum
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Gene info
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Identity
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Gene
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Long gene name
serum response factor
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1995-02-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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