OVA Conjugated Reverse Triiodothyronine (rT3)
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Catalog number
CPC022Ge21
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Price
Please ask
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Size
200ug
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Source
Protein conjugation
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Predicted Molecular Mass KD
651.0Da
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Accurate Molecular Mass KD
NA
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Organism species
Pan-species (General)
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Item Name
Reverse Triiodothyronine
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Alternative Names
RT3; r-T3; Reverse T3;(2S)-2-Amino-3-[4-(4-Hydroxy-3,5-Diiodophenoxy)-3-Iodophenyl]propanoic Acid; 3,3',5'-Triiodo-L-thyronine
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Research Area
Infection immunity;Endocrinology;Hormone metabolism;Autoimmunity;
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Buffer Formulation
PBS, pH7.4.
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Traits
Freeze-dried powder
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Purity
>95%
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Protein length
NA
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Tag
No tag
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Expression System
Protein Conjugation
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Application
Immunogen; SDS-PAGE; WB
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Gene
Ovalbumin (abbreviated OVA) is the main protein found in egg white, making up 60-65% of the total protein. Ovalbumin displays sequence and three-dimensional homology to the serpin superfamily, but unlike most serpins it is not a serine protease inhibitor. The function of ovalbumin is unknown, although it is presumed to be a storage protein. OVA is also the best characterized and the first antigen proteins used as a transgene to make transgenic mice. Many different transgenic mouse models have systemic OVA expression driven by the ubiquitously expressed b-actin promoter or tissue-specific OVA expression with insulin promoter to drive the transgene expression, for studying type I diabetes, or in different isoforms, secreted or cell-membrane associated, and more recently as inducible transgene models. These C57BL/6 mice, BALB/c mice models are well characterized, and have contributed to our understanding of immunogenicity and tolerance by the OVA model.
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Description
Reverse transcription primers are used in PCR but in vivo reverse transcription begins when the viral particle that enters the cytoplasm of a target cell with its reverse transcriptase. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized. The process of reverse transcription generates, in the cytoplasm, a linear DNA via an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA . Extant models for reverse transcription propose that two specialized template switches known as strand-transfer reactions or “jumps” are required to generate the LTRs.
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Goup
reverse transcription
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Gene target
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Short name
OVA Conjugated Reverse Triiodothyronine (rT3)
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Technique
reverse, antibody Conjugates
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Host
chemical synthesis
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Species
General
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Alternative name
OVA coupled Reverse Triiodothyronine (rT3)
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Alternative technique
transctription
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MeSH Data
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Name
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Concept
Scope note:
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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