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Description
A Rabbit polyclonal antibody against Human, Mouse, Pig LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1). This antibody is labeled with FITC.
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Specifications
Host: Rabbit; Species Reactivity: Human, Mouse, Pig; Clonality: polyclonal; Tested applications: WB, IHC; Concentration: 500ug/ml; Isotype: IgG; Conjugation: FITC
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Additional_information
Sequence of the immunogen: LIMS1 (Ala68~Cys303); Buffer composition: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Storage_and_shipping
Upon receipt, store at -20°C or -80°C. Prepare working aliqotes prior to storage to avoid repeated freeze-thaw cycles.
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Notes
Research Use Only.
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Properties
If you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Cloud Clone Corp Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cloud Clone Corp FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional description
Antigens are peptides or recombinant or native dependent on the production method. For cells, cell lines and tissues in culture till half confluency.
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Conjugation
Anti-FITC Antibody
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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About
Pigs and the smaller guinea pigs are frequent used as models for humans. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Group
Polyclonals and antibodies
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French translation
anticorps
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Gene target
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Gene symbol
LIMS1-AS1, PDLIM5, LIMS1, LHX1
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Short name
LIM Senescent Antigen Like Domains Protein 1 (LIMS1) Polyclonal Antibody ( , Mouse, ), FITC
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Technique
Polyclonal, Antibody, antigen, Mouse, FITC, antibodies against human proteins, antibodies for, antigenes, Fluorescein, mouses, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
mouse
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Label
FITC
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Species
Pig, Humans, Mouses, Pigs and Guinea pigs
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Alternative name
LIM And Senescent cellular protein Like Domains Protein 1 (LIM and senescent cellular antigen-like-containing domain protein 3; LIM and senescent cellular antigen-like-containing domain protein 3-like; Uncharacterized protein; complementary Desoxyribonucleic acid FLJ59124, highly similar to Particularly interesting newCys-histidine protein; complementary Desoxyribonucleic acid, FLJ79109, highly similar to Particularly interesting newCys-histidine protein) polyclonal (antibody to-) (H. sapiens, Mouse, swine), fluorecein
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Alternative technique
polyclonals, antibodies, antigenes, murine, fluorescine
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Alternative to gene target
LIM and senescent cell antigen-like-containing domain protein 3; LIM and senescent cell antigen-like-containing domain protein 3-like; Uncharacterized protein; cDNA FLJ59124, highly similar to Particularly interesting newCys-His protein; cDNA, FLJ79109, highly similar to Particularly interesting newCys-His protein, LIMS1 and IDBG-544075 and ENSG00000257207 and, zinc ion binding, Cytoplasm
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Tissue
cell
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