Islet Cell Autoantigen 1 (ICA1) Monoclonal Antibody (Human, Rat), FITC

  • Catalog number
    MAC538Hu21-1ml-FITC
  • Price
    Please ask
  • Size
    1ml
  • Description
    A Mouse monoclonal antibody against Human, Rat Islet Cell Autoantigen 1 (ICA1). This antibody is labeled with FITC.
  • Specifications
    Host: Mouse; Species Reactivity: Human, Rat; Clonality: monoclonal; Tested applications: WB, IHC; Concentration: 1mg/mL; Isotype: IgG2b Kappa; Conjugation: FITC
  • Additional_information
    Sequence of the immunogen: Met1~Gly258; Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • Storage_and_shipping
    Upon receipt, store at -20°C or -80°C. Prepare working aliqotes prior to storage to avoid repeated freeze-thaw cycles.
  • Notes
    Research Use Only.
  • Properties
    If you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Cloud Clone Corp Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cloud Clone Corp FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Additional description
    For cells, cell lines and tissues in culture till half confluency.
  • Conjugation
    Anti-FITC Antibody
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Latin name
    Rattus norvegicus
  • French translation
    anticorps
  • Gene target
  • Gene symbol
    ICA1-AS1, ICA1
  • Short name
    Islet Autoantigen 1 (ICA1) Monoclonal Antibody ( , ), FITC
  • Technique
    Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies
  • Host
    Rat
  • Label
    FITC
  • Species
    Rat, Humans, Rats
  • Alternative name
    Islet cellular Autoantigen 1 (islet cell autoantigen 1, 69kDa) monoclonal (antibody to-) (H. sapiens, Rat), fluorecein
  • Alternative technique
    antibodies, fluorescine
  • Alternative to gene target
    islet cell autoantigen 1, 69kDa, ICA69 and ICAp69, ICA1 and IDBG-8551 and ENSG00000003147 and 3382, protein domain specific binding, Cell surfaces, Ica1 and IDBG-128452 and ENSMUSG00000062995 and 15893, BT.35115 and IDBG-629525 and ENSBTAG00000000799 and 535346
  • Tissue
    cell
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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