Immunoglobulin G (IgG) Polyclonal Antibody (Rat), HRP

• Catalog number
  PAA544Ra01-1ml-HRP
• Price
  Please ask
• Size
  1ml

• Description
  A Rabbit polyclonal antibody against Rat Immunoglobulin G (IgG). This antibody is labeled with HRP.
• Specifications
  Host: Rabbit; Species Reactivity: Rat; Clonality: polyclonal; Tested applications: WB; IHC; ICC; IP.; Concentration: 500ug/ml; Isotype: IgG; Conjugation: HRP
• Additional_information
  Sequence of the immunogen: Full Length IgG of Rat Plasma; Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
• Storage_and_shipping
  Upon receipt, store at -20°C or -80°C. Prepare working aliqotes prior to storage to avoid repeated freeze-thaw cycles.
• Notes
  Research Use Only.
• Properties
  If you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
• Conjugation
  HRP Conjugated
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Group
  Polyclonals and antibodies
• Latin name
  Rattus norvegicus
• French translation
  anticorps

• Gene target
  Immunoglobulin  
• Gene symbol
  IGHV3-71, SEMA4D, FCGR2A
• Short name
  Immunoglobulin G (IgG) Polyclonal Antibody ( ),
• Technique
  Polyclonal, Antibody, IgG, antibodies against human proteins, antibodies for, IgGs, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Host
  Rat
• Isotype
  IgG, IgG
• Label
  HRP
• Species
  Rat, Rats
• Alternative name
  Immunoglobulin G (Immunoglobulin G) polyclonal (antibody to-) (Rat), horseradish peroxidase
• Alternative technique
  polyclonals, antibodies, immunoglobulins

Gene info

• Identity
  HGNC:5621
• Gene
  IGHV3-71
• Long gene name
  immunoglobulin heavy variable 3-71 (pseudogene)
• Synonyms gene
  • IGHV3-G
• Synonyms gene name
  • immunoglobulin heavy variable 3-71
  • immunoglobulin heavy variable 3-G pseudogene (provisional)
  • immunoglobulin heavy variable 3-G (provisional)
  • immunoglobulin heavy variable 3-71 pseudogene
• Synonyms
  • IGHV3-g
• GenBank acession
  • AB019437
• Locus
  14q32.33
• Discovery year
  2000-04-04
• Entrez gene record
  28411
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000151881

Gene info

• Identity
  HGNC:10732
• Gene
  SEMA4D
• Long gene name
  semaphorin 4D
• Synonyms gene
  • SEMAJ
  • C9orf164
• Synonyms gene name
  • chromosome 9 open reading frame 164
  • sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
• Synonyms
  • CD100
  • coll-4
  • FLJ39737
• Synonyms name
  • M-sema G
• GenBank acession
  • U60800
• Locus
  9q22.2
• Discovery year
  1999-06-25
• Entrez gene record
  10507
• Pubmed identfication
  • 8876214
  • 8969198
• RefSeq identity
  • NM_006378
• Classification
  • Immunoglobulin like domain containing
  • Semaphorins
  • CD molecules
• VEGA ID
  OTTHUMG00000020185

Gene info

• Identity
  HGNC:3616
• Gene
  FCGR2A
• Long gene name
  Fc gamma receptor IIa
• Synonyms gene
  • FCG2
  • FCGR2A1
  • FCGR2
• Synonyms gene name
  • Fc fragment of IgG, low affinity IIa, receptor for (CD32)
  • Fc fragment of IgG, low affinity IIa, receptor (CD32)
  • Fc fragment of IgG receptor IIa
• Synonyms
  • CD32
  • CD32A
  • IGFR2
  • CDw32
  • Fc-gamma-RIIa
  • FcgammaRIIa
• Synonyms name
  • Immunoglobulin G Fc receptor II
• GenBank acession
  • J03619
• Locus
  1q23.3
• Discovery year
  1988-11-30
• Entrez gene record
  2212
• Pubmed identfication
  • 2139735
  • 8994865
• RefSeq identity
  • NM_021642
• Classification
  • Immunoglobulin like domain containing
  • CD molecules
• VEGA ID
  OTTHUMG00000034469
• Locus Specific Databases
  • LRG_1109

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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