Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) Polyclonal Antibody (Human), FITC
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Catalog number
PAB230Hu01-100ul-FITC
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Price
Please ask
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Size
100ul
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Description
A Rabbit polyclonal antibody against Human Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4). This antibody is labeled with FITC.
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Specifications
Host: Rabbit; Species Reactivity: Human; Clonality: polyclonal; Tested applications: WB, IHC; Concentration: 500ug/ml; Isotype: IgG; Conjugation: FITC
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Additional_information
Sequence of the immunogen: CTLA4 (Gly52~Cys211); Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
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Storage_and_shipping
Upon receipt, store at -20°C or -80°C. Prepare working aliqotes prior to storage to avoid repeated freeze-thaw cycles.
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Notes
Research Use Only.
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Properties
If you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Cloud Clone Corp Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cloud Clone Corp FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional description
Antigens are peptides or recombinant or native dependent on the production method.
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Conjugation
Anti-FITC Antibody
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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French translation
anticorps
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Gene target
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Gene symbol
CTLA4
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Short name
Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) Polyclonal Antibody ( ), FITC
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Technique
Polyclonal, Antibody, antigen, FITC, antibodies against human proteins, antibodies for, antigenes, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Label
FITC
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Species
Human, Humans
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Alternative name
Cytotoxic T-Lymphocyte Associated protein 4 (cytotoxic T-lymphocyte-associated protein 4) polyclonal (antibody to-) (H. sapiens), fluorecein
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Alternative technique
polyclonals, antibodies, antigenes, fluorescine
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Alternative to gene target
cytotoxic T-lymphocyte-associated protein 4, CD and CD152 and CELIAC3 and CTLA-4 and GRD4 and GSE and IDDM12, CTLA4 and IDBG-79215 and ENSG00000163599 and 1493, protein binding, Cell surfaces, Ctla4 and IDBG-159662 and ENSMUSG00000026011 and 12477, CTLA-4 and IDBG-643375 and ENSBTAG00000013170 and 281732
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Tissue
lymphocyte
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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