Breast Cancer Susceptibility Protein 1 (BRCA1) Polyclonal Antibody (Mouse), FITC
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Catalog numberPAB376Mu01-200ul-FITC
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Price:
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Size200ul
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DescriptionA Rabbit polyclonal antibody against Mouse Breast Cancer Susceptibility Protein 1 (BRCA1). This antibody is labeled with FITC.
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SpecificationsHost: Rabbit; Species Reactivity: Mouse; Clonality: polyclonal; Tested applications: WB, IHC; Concentration: 500ug/ml; Isotype: IgG; Conjugation: FITC
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Additional_informationSequence of the immunogen: BRCA1 (Phe1524~Leu1781); Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
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Storage_and_shippingUpon receipt, store at -20°C or -80°C. Prepare working aliqotes prior to storage to avoid repeated freeze-thaw cycles.
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NotesResearch Use Only.
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PropertiesIf you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Cloud Clone Corp Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cloud Clone Corp FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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French translationanticorps
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Gene target
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Gene symbolBRCA1, BRCA1P1
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Short nameBreast Cancer Susceptibility Protein 1 (BRCA1) Polyclonal Antibody (Mouse), FITC
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TechniquePolyclonal, Antibody, Mouse, FITC, antibodies against human proteins, antibodies for, Fluorescein, mouses, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Hostmouse
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LabelFITC
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SpeciesMouse, Mouses
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Alternative nameBreast Cancer Susceptibility Protein 1 (breast cancer 1, early onset) polyclonal (antibody to-) (Mouse), fluorecein
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Alternative techniquepolyclonals, antibodies, murine, fluorescine
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Alternative to gene targetbreast cancer 1, early onset, BRCAI and BRCC1 and BROVCA1 and IRIS and PNCA4 and PPP1R53 and PSCP and RNF53, BRCA1 and IDBG-52172 and ENSG00000012048 and 672, androgen receptor binding, nuclei, Brca1 and IDBG-212218 and ENSMUSG00000017146 and 12189, BRCA1 and IDBG-640630 and ENSBTAG00000022520 and 353120
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Tissuebreast
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Diseasecancer
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Gene info
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Identity
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Gene
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Long gene nameBRCA1 DNA repair associated
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Synonyms gene name
- breast cancer 1, early onset
- breast cancer 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1991-02-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ring finger proteins
- Protein phosphatase 1 regulatory subunits
- BRCA1 A complex
- BRCA1 B complex
- BRCA1 C complex
- FA complementation groups
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameBRCA1 pseudogene 1
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Synonyms
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Synonyms name
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Locus
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Discovery year2005-05-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data