Bcl2 Associated X Protein (Bax) Polyclonal Antibody (Pig), FITC
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Catalog number
PAB343Po01-1ml-FITC
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Price
Please ask
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Size
1ml
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Description
A Rabbit polyclonal antibody against Pig Bcl2 Associated X Protein (Bax). This antibody is labeled with FITC.
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Specifications
Host: Rabbit; Species Reactivity: Pig; Clonality: polyclonal; Tested applications: WB; Concentration: 500ug/ml; Isotype: IgG; Conjugation: FITC
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Additional_information
Sequence of the immunogen: Bax (Met1~Gly192); Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
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Storage_and_shipping
Upon receipt, store at -20°C or -80°C. Prepare working aliqotes prior to storage to avoid repeated freeze-thaw cycles.
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Notes
Research Use Only.
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Properties
If you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Cloud Clone Corp Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cloud Clone Corp FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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About
Pigs and the smaller guinea pigs are frequent used as models for humans. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Group
Polyclonals and antibodies
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French translation
anticorps
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Gene target
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Gene symbol
BAX
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Short name
Bcl2 Associated X Protein (Bax) Polyclonal Antibody ( ), FITC
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Technique
Polyclonal, Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Label
FITC
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Species
Pig, Pigs and Guinea pigs
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Alternative name
B-cellular CLL/lymphoma 2 Associated X Protein (BCL2-associated X protein) polyclonal (antibody to-) (swine), fluorecein
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Alternative technique
polyclonals, antibodies, fluorescine
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Alternative to gene target
B-cell CLL/lymphoma 2, Bcl-2 and PPP1R50, BCL2 and IDBG-4354 and ENSG00000171791 and 596, protein phosphatase 2A binding, nuclei, Bcl2 and IDBG-185673 and ENSMUSG00000057329 and 12043, BCL2 and IDBG-645006 and ENSBTAG00000019302 and 281020
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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