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Shipping
The Shipping of mouse anti-E. coli and LPS IGM monoclonal antibody, clone 3H11 is in gel packs
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Storage
The storage of mouse anti-E. coli and LPS IGM monoclonal antibody, clone 3H11 must be at - 20 C
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Protocol
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Category
Antibody
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Clonality
Monoclonal
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Ckross Reactivity
NA
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Host Origin
Mouse
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Applications
mouse anti-E. coli and LPS IGM monoclonal antibody, clone 3H11 is used for : Enzym Linked Immuno-Sorbent Assay and Western Blot
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Concentration
1 mg/mL
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Immunogen
Staphylococcal enterotoxin B (SEB)
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Purity
mouse anti-E. coli and LPS IGM monoclonal antibody, clone 3H11 is purified by affinity chromatography.
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Stability
The shelf life of mouse anti-E. coli and LPS IGM monoclonal antibody, clone 3H11 is a maximum one year
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by Chondrex they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
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Gene
Bacterial pathogen lipopolysaccharides (LPS) are the major outer surface membrane components present in almost all Gram-negative bacteria and act as extremely strong stimulators of innate or natural immunity in diverse eukaryotic species ranging from insects to humans. LPS consist of a poly- or oligosaccharide region that is anchored in the outer bacterial membrane by a specific carbohydrate lipid moiety termed lipid A. The lipid A component is the primary immunostimulatory center of LPS. With respect to immunoactivation in mammalian systems, the classical group of strongly agonistic (highly endotoxin) forms of LPS has been shown to be comprised of a rather similar set of lipid A types. In addition, several natural or derivative lipid A structures have been identified that display comparatively low or even no immunostimulation for a given mammalian species. Some members of the latter more heterogeneous group are capable of antagonizing the effects of strongly stimulatory LPS/lipid A forms. Agonistic forms of LPS or lipid A trigger numerous physiological immunostimulatory effects in mammalian organisms, but--in higher doses--can also lead to pathological reactions such as the induction of septic shock. Cells of the myeloid lineage have been shown to be the primary cellular sensors for LPS in the mammalian immune system. During the past decade, enormous progress has been obtained in the elucidation of the central LPS/lipid A recognition and signaling system in mammalian phagocytes. According to the current model, the specific cellular recognition of agonistic LPS/lipid A is initialized by the combined extracellular actions of LPS binding protein (LBP), the membrane-bound or soluble forms of CD14 and the newly identified Toll-like receptor 4 (TLR4)*MD-2 complex, leading to the rapid activation of an intracellular signaling network that is highly homologous to the signaling systems of IL-1 and IL-18. The elucidation of structure-activity correlations in LPS and lipid A has not only contributed to a molecular understanding of both immunostimulatory and toxic septic processes, but has also re-animated the development of new pharmacological and immuno-stimulatory strategies for the prevention and therapy of infectious and malignant diseases.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps
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Gene target
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Gene symbol
IRF6
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Short name
mouse anti- LPS IGM monoclonal antibody, clone 3H11
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Technique
Antibody, anti-, Mouse, anti, IgM, antibody to, antibodies, antibodies against human proteins, antibodies for, IgMs, Monoclonals or monoclonal antibodies, mouses
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Host
mouse, Escherichia coli, Recombination of bioactive proteins and longer peptides in Escherichia Coli is done often with His tagging. Chondrex supplies Rec. E. Coli affinity purified or tag purified antigens in 1 quantities or bulk volumes on request.
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Isotype
IGM, IgM
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Species
E. coli, E. coli, Mouses
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Alternative name
mouse Antibody toE. coli and LPS Immunoglobulin M monoclonal (antibody to-), clonality 3H11
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Alternative technique
antibodies, murine, escherichia, immunoglobulin-m
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Clone name
3H11
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