OxiSelect™ Hydrogen Peroxide/Peroxidase Assay test kit (Fluorimetry), Trial Size
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Catalog numberSTA-344-T
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Price:
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Size50 assays
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We send this reagant onRoom temperature
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The temperatureerature for storage isUpon receipt, aliquot and store the ADHP probe, HRP at -20ºC. Avoid multiple freeze/thaw cycles. Store the remaining test kit components at 4ºC. Protect ADHP from light.
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SDS or safty data sheetmore (STA-344-T) GHS.pdf
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Type of reagentOxidative Stress/Damage
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Related techniquesOxidative Stress/ Damage, Reactive Oxygen Species (ROS) Assays, Hydrogen Peroxide and Peroxidase Assays
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Use of the reagentThe OxiSelect™ Hydrogen Peroxide/Peroxidase Assay test kit is a sensitive quantitative Fluorimetry assay for hydrogen peroxide or peroxidase. In the presence of HRP, ADHP reacts with H2O2 in a 1:1 stoichiometry to produce highly fluorescent Resorufin. The Resorufin product can be easily read by a fluorescence microplate reader with an excitation of 530-560 nm and an emission of 590 nm, or for absorbance at 560 nm. Fluorescence values are proportional to the H2O2 or peroxidase levels within the samples. The H2O2 or peroxidase content in unknown samples is determined by comparison with its respective standard curve.
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Cellular useDetect hydrogen peroxide concentrations as low as 50 nM
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Protocol informationSuitable for use with cell lysates, tissue homogenates, cell culture supernatants, plasma, serum, urine, or other biological fluids
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Reagent propertiesSimple assay protocol provides results in 30-90 minutes, depending on sample type
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Species compatible toHumans
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Use of this test kit or productGenetics lab
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Antigena specific protein sequence
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BufferThe concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,92
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Molecular weightin kilo Danlto or kDa
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This reagent is purified0.97
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TestCellular biology labs supplies other types of Assays as 1.
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ConjugatePeroxidases with EC number 1.11.1.x are catalyzing the reaction of ROOR' + electron donor (2 e−) + 2H+ → ROH + R'OH
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Gene target
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Short nameOxiSelect™ Hydrogen Peroxide/ Assay test kit (Fluorimetry), Trial Size
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TechniqueAssay, assays
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HostAssay
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Labelperoxidase
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Alternative nameOxiSelect™ Hydrogen Peroxide/Peroxidase test test reagent (Fluorimetry), Trial Size
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Alternative techniquearrays, tests, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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MeSH Data
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Name
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ConceptScope note: Radioimmunoassay of proteins using antibody coupled to an immunosorbent.
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Tree numbers
- E05.478.566.380.830
- E05.478.566.639.830
- E05.601.470.380.830
- E05.601.470.639.830
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Qualifiersethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data