NAD+/NADH Assay test kit (Fluorimetry)

We send this reagant on

Blue Ice
The temperatureerature for storage is

Upon receipt, store the NAD Cycling Enzyme and NAD+ Standard at -80°C. Store the Fluorimetry Probe and Electron Mediator at -20°C. Store the remaining components at 4°C.
Technical file
SDS or safty data sheet

more (MET-5030) GHS.pdf
Type of reagent

Metabolism Research
Related techniques

Metabolism Research, Cellular Metabolism, NAD+/NADH Assay
Use of the reagent

Our NAD+/NADH Assay test kit detects NAD+ and NADH in cell and tissue lysates. Total NAD+/NADH can be detected or samples can be treated with an acid or base treatment to specifically detect NAD+ or NADH. This assay uses an enzymatic cycling reaction that reduces NAD+ to NADH, which then reacts with a Fluorimetry probe and is detected with a fluorescence plate reader at 530-570nm excitation and 590-600nm emission. NAD+/NADH levels in unknown samples are calculated based on the provided NAD+ standard curve.
Cellular use

Detects NAD+, NADH, or total NAD+/NADH
Protocol information

Compatible with cell or tissue lysates
Reagent properties

NAD+ standard included
Conjugation and form

host specific
Species compatible to

human cells and cell lines and other cells
Use of this test kit or product

Molecular biology
Antigen

the sequence of the protein
Buffer

The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,13
Molecular weight

kiloDalton
This reagent is purified

0.98
Test

Cellular biology labs supplies other types of Assays as 1.

Gene target

NAD+/NADH test kit Fluorimetry
Short name

NAD+/NADH Assay test kit (Fluorimetry)
Technique

Assay, assays
Host

Assay
Alternative name

Nicotinamide adenine dinucleotide+/NADH test test reagent (Fluorimetry)
Alternative technique

arrays, tests, kits
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832