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We send this reagant on
Blue Ice
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The temperatureerature for storage is
The LDH Cytotoxicity Assay Reagent is a clear, slightly red, ready-to-use solution. Aliquot as needed to avoid repeated freeze-thaw cycles and store at -20°C protected from light. If precipitates or turbidity are observed upon thawing, warm the solution to 37°C for 5-10 minutes and agitate to dissolve the precipitates. Store the Triton X-100 Solution at Room temperatureerature.
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SDS or safty data sheet
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Type of reagent
Cell Based Assays
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Related techniques
Cell Based Assays, Cell Health Assays >Cytotoxicity Assays, LDH Cytotoxicity Assay
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Use of the reagent
Cell Biolabs’ CytoSelect™ LDH Cytotoxicity Assay test kit provides a colorimetry format for measuring and monitoring cell cytotoxicity. The test kit contains sufficient reagents for the evaluation of 960 assays in 96-well plates. Cells can be plated and then treated with compounds or agents that affect cell viability. Upon cell death, lactate dehydrogenase (LDH), a soluble enzyme found in the cytoplasm, is released into the growth media. The growth media is then transferred to another plate and the released LDH is then detected with cytotoxicity reagent. In the presence of lactate substrate (included in the LDH Cytotoxicity Reagent) LDH converts lactate to pyruvate and generates nicotinamide adenine dinucleotide (NADH). The WST-1 molecule, also present in the LDH Cytotoxicity Reagent, is converted from WST-1 to the orange formazan form. An increase in cell cytotoxicity is accompanied by increased LDH release and increased colorimetry signal. The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues, depending on LDH expression levels. The LDH Cytotoxicity Reagent can be used to detect cytotoxicity in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells.
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Cellular use
LDH Cytotoxicity Reagent can be used to detect cytotoxicity in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells
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Protocol information
Cells can be plated and then treated with compounds or agents that affect cell viability
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Reagent properties
colorimetry test kit, containing sufficient reagents for the evaluation of 960 assays in 96-well plates
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Living modified organisme in this reagent or LMO
Not applicable
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Conjugation and form
host specific
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Species compatible to
human cells and cell lines and other cells
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Use of this test kit or product
Molecular biology
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Antigen
the sequence of the protein
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Buffer
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,45
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Molecular weight
kiloDalton
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This reagent is purified
0.98
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Test
Cellular biology labs supplies other types of Assays as 1.
