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We send this reagant on
Blue Ice
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The temperatureerature for storage is
CytoSelect™ Cell Proliferation Assay Reagent (colorimetry) is a clear, slightly red, ready-to-use solution and should be stored at 4°C. If precipitates or turbidity are observed upon thawing, warm the solution to 37°C for 5–10 minutes and agitate to dissolve the precipitates. For longer periods, aliquot and store at –20°C protected from light to avoid repeated freeze-thaw cycles.
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SDS or safty data sheet
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Type of reagent
Cell Based Assays
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Related techniques
Cell Based Assays, Cell Health Assays > Cell Proliferation Assays
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Use of the reagent
Cell Biolabs’ CytoSelect™ WST-1 Cell Proliferation Assay Reagent provides a colorimetry format for measuring and monitoring cell proliferation. The 10 mL volume is sufficient for the evaluation of 960 assays in ten 96-well plates or 192 assays in eight 24-well plates. Cells can be plated and then treated with compounds or agents that affect proliferation. Cells are then detected with the proliferation reagent, which is converted in live cells from WST-1 to the formazan form in the presence of cellular NADH and an electron mediator. An increase in cell proliferation is accompanied by increased signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions. The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues. This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells.
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Cellular use
Contains sufficient reagents for the evaluation of 960 assays in ten 96-well plates or 192 assays in eight 24-well plates
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Protocol information
Can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues
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Reagent properties
Cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells
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Conjugation and form
host specific
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Species compatible to
human cells and cell lines and other cells
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Use of this test kit or product
Molecular biology
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Antigen
the sequence of the protein
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Buffer
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,49
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Molecular weight
kiloDalton
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This reagent is purified
0.98
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Test
Cellular biology labs supplies other types of Assays as 1.
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Description
For cells, cell lines and tissues in culture till half confluency.
