CytoSelect™ 24-well Anoikis Assay (colorimetry/Fluorimetry)
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Catalog number
CBA-080
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Price
Please ask
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Size
24 assays
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We send this reagant on
Room temperature
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The temperatureerature for storage is
Store the Calcein AM and Ethidium Homodimer at -20ºC. Store all other components at 4ºC.
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SDS or safty data sheet
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Type of reagent
Cell Based Assays
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Related techniques
Cell Based Assays, Cell Health Assays, Anoikis (Anchorage-Dependent Cell Death)
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Use of the reagent
Adhesion to the extraceullular matrix is essential for the survival and propagation of many adherent cells. Apoptosis resulting from the loss of adhesion to the ECM is known as anoikis. Anoikis is involved in the physiological processes of tissue renewal and cell homeostasis. Our CytoSelect™ Anoikis Assays allow you to quantify and monitor anoikis in cells using a precoated plate. Live cells can be viewed under a microscope and quantified on a plate reader by MTT (colorimetry) or Calcein AM (Fluorimetry); both reagents are included in the test kit. Dead cells are detected with the red EthD-1 reagent, also included.
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Cellular use
Detect live and dead cells by microscopy, fluorescence, or flow cytometry
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Protocol information
Quantify live cells on a standard microplate reader or fluorometer
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Reagent properties
Precoated plate included
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Conjugation and form
host specific
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Species compatible to
human cells and cell lines and other cells
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Use of this test kit or product
Molecular biology
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Antigen
the sequence of the protein
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Buffer
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,57
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Molecular weight
kiloDalton
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This reagent is purified
0.98
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Test
Cellular biology labs supplies other types of Assays as 1.
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