AAV-DJ/8 Helper Free Expression System
#
-
Catalog numberVPK-410-DJ-8
-
Price:
-
Size1 test kit
-
-
We send this reagant onBlue Ice
-
The temperatureerature for storage is-20ÂșC
-
SDS or safty data sheetmore (VPK-410 through VPK-430-SER6) GHS.pdf
-
Type of reagentViral Expression
-
Related techniquesViral Expression, Adeno-Associated Virus (AAV) Expression, AAV Helper Free Complete Expression Systems, AAV-DJ/8 Helper Free Complete Expression Systems
-
Use of the reagentThe AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2. AAV-DJ/8 was created by making a point mutation in the heparin binding domain of AAV-DJ. Recombinant AAV produced from the AAV-DJ/8 system closely resembles AAV-8 and AAV-9 in its ability to infect heart and brain tissues.
-
Cellular useComplete Expression System contains packaging plasmids, expression vector and GFP control vector
-
Protocol informationpHelper plasmid contains required E2A, E4, and VA RNA adenoviral genes; eliminates the need for a helper adenovirus
-
Reagent propertiesAAV-DJ/8 is a mutant of AAV-DJ designed to provide increased uptake in brain tissue, closely mimicking AAV-8 and AAV-9
-
Additional lab adviceCloning capacity of expression vector = 3 kb
-
Living modified organisme in this reagent or LMONot applicable
-
Species compatible toHumans
-
Use of this test kit or productGenetics lab
-
Antigena specific protein sequence
-
BufferThe concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,94
-
Molecular weightin kilo Danlto or kDa
-
This reagent is purified0.97
-
Gene target
-
Gene symbolAAVS1, PATE3, PARK7, DNAJA1
-
Short nameAAV-DJ/8 Helper Free Expression System
-
Alternative nameAAV-DJ/8 Helper Free Expression System
-
Gene info
-
Identity
-
Gene
-
Long gene nameadeno-associated virus integration site 1
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year1990-09-10
-
Entrez gene record
-
Pubmed identfication
Gene info
-
Identity
-
Gene
-
Long gene nameprostate and testis expressed 3
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2008-12-17
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- PATE family
- LY6/PLAUR domain containing
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameParkinsonism associated deglycase
-
Synonyms gene name
- Parkinson disease (autosomal recessive, early onset) 7
- parkinson protein 7
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2001-08-07
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Glutamine amidotransferase class 1 domain containing
-
VEGA ID
-
Locus Specific Databases
Gene info
-
Identity
-
Gene
-
Long gene nameDnaJ heat shock protein family (Hsp40) member A1
-
Synonyms gene
-
Synonyms gene name
- DnaJ (Hsp40) homolog, subfamily A, member 1
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year1998-03-26
-
Entrez gene record
-
Pubmed identfication
-
Classification
- DNAJ (HSP40) heat shock proteins
-
VEGA ID