HIF-1 Alpha DNA Binding Activity Assay Kit

Shipping Temp

Room Temp
Storage Temp

Upon receipt, store the Biotinylated Double Stranded DNA and Anti-HIF-1 Alpha Antibody at -20ºC. Store all other components at 4ºC.
Detection Method

Colorimetric
Category Product Type

Kit
URL link to datasheet
URL link to SDS

more (CBA-282) GHS.pdf
UNSPSC

41116158
Product Line

Cell Based Assays
Short Description

Cell Biolabs’ HIF-1 Alpha DNA Binding Activity Assay Kit is an immunoassay developed for rapid detection of activated HIF-1 in any protein sample. Active HIF-1 complex is captured on a double stranded oligonucleotide containing an HRE that is attached to the well. HIF-1 Alpha is then detected with an anti-HIF-1 Alpha antibody followed by an HRP conjugated secondary antibody. Each kit provides sufficient reagents to perform up to a total of 96 assays, and can detect HIF-1 Alpha from human, mouse, or rat. The HIF-1 DNA Binding Activity Assay takes only 4.5 hours to complete compared to a standard 3 day electromobility shift (EMSA) method.
Description

The HIF-1 Alpha DNA Binding Activity Assay Kit is a α- or alpha protein sometimes glycoprotein present in blood.
Test

Cell Biolabs supplies other types of Assays as 1.

Gene target

HIF-1 Alpha DNA Binding Activity Kit
Gene symbol

HIF1A, SETD2, ARNT, PRKDC, HLA-DOA, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1
Short name

HIF-1 Alpha DNA Binding Activity Assay Kit
Technique

Assay, dna, assays
Host

Assay
Alternative name

HIF-1 a Desoxyribonucleic acid Binding Activity test reagent
Alternative technique

arrays, kits
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Identity

HGNC:4910
Gene

HIF1A
Long gene name

hypoxia inducible factor 1 subunit alpha
Synonyms gene name
- hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Synonyms
- MOP1
- HIF-1alpha
- PASD8
- HIF1
- bHLHe78
Synonyms name
- PAS domain-containing protein 8”, “Class E basic helix-loop-helix protein 78”, “Member of PAS protein 1
GenBank acession
- U22431
Locus

14q23.2
Discovery year

1995-03-27
Entrez gene record

3091
Pubmed identfication
- 8786149
- 9079689
RefSeq identity
- NM_001530
Classification
- Basic helix-loop-helix proteins
VEGA ID

OTTHUMG00000140344

Identity

HGNC:5670
Gene

IGHVII-1-1
Long gene name

immunoglobulin heavy variable (II)-1-1 (pseudogene)
Synonyms gene name
- immunoglobulin heavy variable (II)-1-1
- immunoglobulin heavy variable (II)-1-1 pseudogene
Synonyms
- IGHV(II)-1-1
GenBank acession
- AB019441
Locus

14q32.33
Discovery year

2000-04-17
Entrez gene record

28377
RefSeq identity
- NG_001019
Classification
- Immunoglobulin heavy locus at 14q32.33
VEGA ID

OTTHUMG00000152325

Identity

HGNC:5760
Gene

IGKV1OR2-1
Long gene name

immunoglobulin kappa variable 1/OR2-1 (pseudogene)
Synonyms gene
- IGKVPZ1
- IGKV1OR-1
Synonyms gene name
- immunoglobulin kappa variable 1/OR-1
- immunoglobulin kappa variable 1/OR-1 pseudogene
- immunoglobulin kappa variable 1/OR-1 (pseudogene)
Synonyms
- IGKV1/OR-1
- IGKV1/OR2-1
GenBank acession
- Z12367
Locus

2p11.1
Discovery year

2000-04-18
Entrez gene record

3531
Pubmed identfication
- 2853098
Classification
- Immunoglobulin kappa (IGK) orphons
VEGA ID

OTTHUMG00000155081