Total protein quantification
Measure total protein concentration in lysates, extracts, purified proteins or biological samples.
Assay Kits
Protein assay kits are used to measure protein concentration, normalize samples and support downstream workflows such as western blotting, ELISA, enzyme assays, purification, cell biology and biochemistry research. They provide standardized reagents, standards and detection systems for estimating total protein or specific protein-related signals. A reliable protein assay helps researchers compare samples fairly, prepare consistent loading amounts and reduce variability across experiments.

Product catalog
Showing 25 unique product names from this subcategory.

Better BCA Protein Assay Kit (Reducing agent compatible)
SKU: SK3051|Size: 500Assays, 500preps

Lowry Protein Assay Kit
SKU: SK4031|Size: 1000Assays, 1000preps

BCA Protein Assay Kit
SKU: SK3021|Size: 500Assays, 500preps

Flag-tag Protein IP Assay Kit (Magnetic Beads)
SKU: K4627-10|Size: 10 Tests

ChIP Assay Kit (Protein A+G Agarose)
SKU: K4625-22|Size: 22 Tests

Bradford Protein Assay Kit
SKU: K4103-1000|Size: 1000 Tests

Protein Assay Kit Kit
SKU: TBS2005|Size: 1000 Tests

JBScreen Thermofluor SPECIFIC Thermal Shift Assay for protein stability
SKU: CS-333|Size: 93 Solutions

Total Protein Assay Kit (With standard, BCA method)
SKU: MBS2548451-01|Size: 96 Tests

The total protein Assay Kit (with standard : BCA method)
SKU: SH0292-1|Size: 48 Tests

BCA Protein Assay Kit II
SKU: K813-5000|Size: 5000 Assays

EZClick™ Myristoylated Protein Assay Kit, Red Fluorescence
SKU: K177-100|Size: 100 Assays

EZClick™ Global Protein Synthesis Assay Kit (FACS/Microscopy), Red Fluorescence
SKU: K715-100|Size: 100 Assays

EZClick™ Palmitoylated Protein Assay Kit (FACS/Microscopy), Red Fluorescence
SKU: K416-100|Size: 100 Assays

Protein Quantification Kit (Bradford Assay)
SKU: MBS9711522-01|Size: 500 Tests

Assay Buffer and Co-factors: Protein MetalloPhosphatase Buffer (10X)
SKU: MBS518004-01|Size: 1 mL

Protein Quantification Kit (BCA Assay)
SKU: MBS9711521-01|Size: 500 Tests

C1q And Tumor Necrosis Factor Related Protein 3 (C1QTNF3) Magnetic Fluorescence Assay Kit
SKU: MBS2161258-01|Size: 96 Tests

DNA Repair Protein RAD50 (RAD50) Magnetic Fluorescence Assay Kit
SKU: MBS2161246-01|Size: 96 Tests

Fas Associating Death Domain Containing Protein (FADD) Magnetic Fluorescence Assay Kit
SKU: MBS2161241-01|Size: 96 Tests

Ring1 And YY1 Binding Protein (RYBP) Magnetic Fluorescence Assay Kit
SKU: MBS2161234-01|Size: 96 Tests

NANOG Homeobox Protein (NANOG) Magnetic Fluorescence Assay Kit
SKU: MBS2161227-01|Size: 96 Tests

Non Imprinted In Prader Willi/Angelman Syndrome Region Protein 1 (NIPA1) Magnetic Fluorescence Assay Kit
SKU: MBS2161224-01|Size: 96 Tests

Ras Association Domain Containing Protein 1 (RASSF1) Magnetic Fluorescence Assay Kit
SKU: MBS2161220-01|Size: 96 Tests

Coiled Coil Domain Containing Protein 80 (CCDC80) Magnetic Fluorescence Assay Kit
SKU: MBS2161219-01|Size: 96 Tests
Overview
Protein quantification is a routine but critical step in many laboratory workflows. Without accurate normalization, differences in western blot bands, enzyme activity or assay signals may reflect unequal loading rather than real biological variation.
Protein assay selection depends on sensitivity, sample buffer, detergent compatibility, reducing agents, detection platform and expected concentration range. Researchers should check whether the assay chemistry is compatible with their extraction buffer.
Applications
Measure total protein concentration in lysates, extracts, purified proteins or biological samples.
Prepare equal protein loading for western blotting, ELISA, enzyme assays or proteomics workflows.
Track protein recovery during purification, concentration or buffer exchange workflows.
Support experiments where protein concentration affects reaction performance or interpretation.
Selection guide
Select a protein assay according to sensitivity, buffer compatibility and downstream workflow.
Choose a kit with a working range that fits your expected protein concentration.
Detergents, reducing agents, chelators and salts may interfere with some protein assay chemistries.
Colorimetric assays are common and simple, while fluorescent methods may offer higher sensitivity.
Prepare standards carefully and choose a standard protein appropriate for your workflow when possible.
Consider whether the result will be used for western blot loading, enzyme activity, purification or normalization.
FAQ
Frequently asked questions about protein quantification kits.
They are used to measure protein concentration and normalize samples before downstream experiments.
Some detergents, reducing agents or salts can interfere with assay chemistry and distort protein measurements.
Choose based on sensitivity, compatibility with your buffer, instrument availability and expected concentration range.
A standard curve converts signal intensity into concentration and helps verify that samples fall within the assay range.
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