HEPES, endotoxin and cell culture tested [CAS# 7365-45-9]

• Catalog number
  H006-100GM
• Price
  Please ask
• Size
  100 g

• Availability
  Please contact us to check current availability
• Long description
  HEPES, endotoxin and cell culture tested [CAS# 7365-45-9]; MW 238.3
• Product format
  Powder
• Product category
  Biochemicals
• Product subcategory
  Buffers and Supplements
• Shipping method
  Ambient temperature
• Storage method
  Store at 2˚ to 8˚C.
• Other products
• Description
  The CAS or CASRN registry number for chemical substances and identifying chemicals by the Chemical Abstract Service is a worldwide used identification of chemicals. This chemical can be supplied in purities of 98% or 95% purity by caissonlab. Bulk in kg or liter volumes on request. For cells, cell lines and tissues in culture till half confluency.
• Buffer
  25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.

• Gene target
  endotoxin   culture   tested   7365-45-9]  
• Gene symbol
  IGHV1-45, SNORD115-45, IGLV5-45, RNU6-9, PIRC45, PIRC47, PIRC38
• Short name
  HEPES
• Technique
  culture
• Alternative name
  HEPES, endotoxin and cellular culture tested [CAS# 7365-45-9]
• Tissue
  cell

Gene info

• Identity
  HGNC:5553
• Gene
  IGHV1-45
• Long gene name
  immunoglobulin heavy variable 1-45
• GenBank acession
  • X92209
• Locus
  14q32.33
• Discovery year
  2000-04-04
• Entrez gene record
  28466
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152075

Gene info

• Identity
  HGNC:33064
• Gene
  SNORD115-45
• Long gene name
  small nucleolar RNA, C/D box 115-45
• Synonyms
  • HBII-52-45
  • SNORD115-48
• Locus
  15q11.2
• Discovery year
  2011-08-22
• Entrez gene record
  100036566
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003498
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:5924
• Gene
  IGLV5-45
• Long gene name
  immunoglobulin lambda variable 5-45
• GenBank acession
  • Z73670
• Locus
  22q11.22
• Discovery year
  2000-05-08
• Entrez gene record
  28781
• RefSeq identity
  • NG_000002
• Classification
  • Immunoglobulin lambda locus at 22q11.2
• VEGA ID
  OTTHUMG00000151054

Gene info

• Identity
  HGNC:34269
• Gene
  RNU6-9
• Long gene name
  RNA, U6 small nuclear 9
• Synonyms
  • U6-9
• Locus
  19p13.3
• Discovery year
  2008-06-10
• Entrez gene record
  101954271
• Pubmed identfication
  • 12711679
• RefSeq identity
  • NR_104080
• Classification
  • Small nuclear RNAs

Gene info

• Identity
  HGNC:37549
• Gene
  PIRC45
• Long gene name
  piwi-interacting RNA cluster 45
• Locus
  9
• Discovery year
  2009-11-05
• Entrez gene record
  100313813
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37551
• Gene
  PIRC47
• Long gene name
  piwi-interacting RNA cluster 47
• Locus
  9
• Discovery year
  2009-11-05
• Entrez gene record
  100313859
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37542
• Gene
  PIRC38
• Long gene name
  piwi-interacting RNA cluster 38
• Locus
  9
• Discovery year
  2009-11-05
• Entrez gene record
  100313788
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

MeSH Data

• Name
  Limulus Test
• Concept
  Scope note: Sensitive method for detection of bacterial endotoxins and endotoxin-like substances that depends on the in vitro gelation of Limulus amebocyte lysate (LAL), prepared from the circulating blood (amebocytes) of the horseshoe crab, by the endotoxin or related compound. Used for detection of endotoxin in body fluids and parenteral pharmaceuticals.
• Tree numbers
  • E01.370.225.124.470
  • E01.370.225.875.150.570
  • E05.091.570
  • E05.200.124.470
  • E05.200.875.150.570
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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