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Target antigen
ZWINT
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Clonality
Polyclonal antibody
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Clone
Polyclonal antibody
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Raised in
rabbit
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Type of the antibody
IgG polyclonal antibody
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Product form
freeze-dried
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Reacts with species
human, mouse, rat
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Analyses
WB,IHC-P
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Immunogen
E.coli-derived human ZWINT recombinant protein (Position: Q29-Q249). Human ZWINT shares 58% and 57.1% amino acid (aa) sequence identity with mouse and rat ZWINT, respectively.
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Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
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Purification
Immunogen affinity purified.
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Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
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Storage condtions
Keep the ZWINT Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The ZWINT Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
ZW10 interactor (Zwint-1) is a protein that in humans is encoded by the ZWINT gene. This gene encodes a protein that is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The encoded protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
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Related articles
1. "Entrez Gene: ZWINT ZW10 interactor". 2. Obuse C, Iwasaki O, Kiyomitsu T, Goshima G, Toyoda Y, Yanagida M (Nov 2004). "A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1". Nature Cell Biology 6 (11): 1135–41. 3. van Vlijmen T, Vleugel M, Evers M, Mohammed S, Wulf PS, Heck AJ, Hoogenraad CC, van der Sluijs P (Aug 2008). "A unique residue in rab3c determines the interaction with novel binding protein Zwint-1". FEBS Letters 582 (19): 2838–42.
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Gene Name
ZWINT
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Protein Name
ZW10 interactor
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Gene Full Name
ZW10 interacting kinetochore protein
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Synonyms
HZwint 1 | HZwint1 | KNTC 2 AP | KNTC2AP | ZWINT 1 | ZWINT | Zwint-1 | ZWINT1 | O95229
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Uniprot ID
O95229
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Entrez GeneID
11130
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps