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Target antigen
TRPC6
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Clonality
Polyclonal antibody
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Clone
Polyclonal antibody
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Raised in
rabbit
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Type of the antibody
IgG polyclonal antibody
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Product form
freeze-dried
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Reacts with species
Human, Mouse, Rat. No cross reactivity with other proteins.
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Analyses
WB,IHC-P
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Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human TRPC6(249-265aa HDYFCKCNDCNQKQKHD), different from the related rat and mouse sequences by three amino acids.
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Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
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Purification
Immunogen affinity purified.
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Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
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Storage condtions
Keep the TRPC6 Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The TRPC6 Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
TRPC6(Transient receptor potential cation channel, subfamily C, member 6), also known as TRP6, is a transient receptor potentialion channel.TRPC6 is a novel member of the TRPC family. The predicted 931-amino acid protein shares 93% identity with mouse Trpc6. Northern blot analysis revealed that TRPC6 is expressed primarily in placenta, lung, spleen, ovary, and small intestine. The TRPC6 gene is mapped on 11q22.1. TRPC6 is a nonselective cation channel that is activated by diacylglycerol(DAG) in a membrane-delimited fashion, independently of protein kinase C. Although TRPC3, the closest structural relative of TRPC6, is activated in the same manner, human TRPC1 and mouse Trpc4 and Trpc5 were unresponsive to DAG. Immunofluorescence studies showed that most TRPC6 expression is confined to podocytes. TRPC6 was also expressed in glomerular endothelial cells. Cardiac-specific overexpression of TRPC6 in transgenic mice resulted in heightened sensitivity to stress, a propensity for lethal cardiac growth and heart failure, and an increase in Nfat-dependent expression of beta-myosin heavy chain(MYH7), a marker for pathologic hypertrophy. Studies of Trpc6 -/- mice showed that Trpc6 has a role in regulation of smooth muscle tone in blood vessels and lung.
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Related articles
1. D'Esposito, M., Strazzullo, M., Cuccurese, M., Spalluto, C., Rocchi, M., D'Urso, M., Ciccodicola, A. Identification and assignment of the human transient receptor potential channel 6 gene TRPC6 to chromosome 11q21-q22. Cytogenet. Cell Genet. 83: 46-47, 1998. 2. Hofmann, T., Obukhov, A. G., Schaefer, M., Harteneck, C., Gudermann, T., Schultz, G. Direct activation of human TRPC6 and TRPC3 channels by diacylglycerol. Nature 397: 259-263, 1999. 3. Kwon, Y., Hofmann, T., Montell, C. Integration of phosphoinositide- and calmodulin-mediated regulation of TRPC6. Molec. Cell 25: 491-503, 2007.
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Gene Name
TRPC6
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Protein Name
Short transient receptor potential channel 6(TrpC6)
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Gene Full Name
transient receptor potential cation channel, subfamily C, member 6
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Synonyms
bZ1P14.9 antibody|FLJ11098 antibody|FLJ14863 antibody|FSGS2 antibody|MTRP6 antibody|Short transient receptor potential channel 6 antibody|si:rp71-1p14.9 antibody|Transient Receptor Potential Cation Channel Subfamily C Member 6 antibody|Transient receptor protein 6 antibody|TRP 6 antibody|TRP-6 antibody|TRP6 antibody|TRPC 6 antibody|TrpC6 antibody|Trpc6 antibody|TRPC6_HUMAN antibody|TRRP6 antibody
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Uniprot ID
Q9Y210
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
