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Target antigen
Thrombospondin
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Clonality
Polyclonal antibody
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Clone
Polyclonal antibody
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Raised in
rabbit
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Type of the antibody
IgG polyclonal antibody
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Product form
freeze-dried
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Reacts with species
human, mouse, rat
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Analyses
WB,ELISA
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Immunogen
E. coli-derived human Thrombospondin recombinant protein (Position: D27-H112). Human Thrombospondin shares 81.4% amino acid (aa) sequence identity with mouse Thrombospondin.
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Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
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Purification
Immunogen affinity purified.
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Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
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Storage condtions
Keep the Thrombospondin Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The Thrombospondin Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
THBS1 is also known as Thrombospondin 1, TSP1. The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. It is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. It has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. In addition, the thrombospondin-1 protein is a member of the thrombospondinfamily. It is a multi-domain matrix glycoprotein that has been shown to be a natural inhibitor of neovascularization and tumorigenesis in healthy tissue. Both positive and negative modulation of endothelial cell adhesion, motility, and growth have been attributed to TSP1. This should not be surprising considering that TSP1 interacts with at least 12 cell adhesion receptors, including CD36, αv integrins, β1 integrins, syndecan, and integrin-associated protein (IAP or CD47). It also interacts with numerous proteases involved in angiogenesis, including plasminogen, urokinase, matrix metalloproteinase,thrombin, cathepsin, and elastase.
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Related articles
1. Blake SM, Strasser V, Andrade N et al. (October 2008). "Thrombospondin-1 binds to ApoER2 and VLDL receptor and functions in postnatal neuronal migration". The EMBO Journal 27 (22): 3069–80. 2. Lopez-Dee ZP, Chittur SV, Patel B, Stanton R, Wakeley M, Lippert B, Menaker A, Eiche B, Terry R, Gutierrez LS (2012)."Thrombospondin-1 type 1 repeats in a model of inflammatory bowel disease: transcript profile and therapeutic effects". PLoS ONE7 (4): e34590. 3. Simantov R, Silverstein RL (September 2003). "CD36: a critical anti-angiogenic receptor". Frontiers in bioscience : a journal and virtual library 8 (1-3): s874–82.
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Gene Name
THBS1
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Protein Name
Thrombospondin-1
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Gene Full Name
thrombospondin 1
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Synonyms
Thrombospondin-1 | Thrombospondin 1 | Thrombospondin1 | THBS1 | TSP | TSP1 | P07996
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Uniprot ID
P07996
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Entrez GeneID
7057
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
