TdT Antibody

• Catalog number
  PB9162
• Price
  Please ask
• Size
  0,1 mg

• Target antigen
  TdT
• Clonality
  Polyclonal antibody
• Clone
  Polyclonal antibody
• Raised in
  rabbit
• Type of the antibody
  IgG polyclonal antibody
• Product form
  freeze-dried
• Reacts with species
  human
• Analyses
  WB
• Immunogen
  E.coli-derived human TdT recombinant protein (Position: K316-A509). Human TdT shares 81% amino acid (aa) sequence identity with mouse TdT.
• Product configuration
  Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Purification
  Immunogen affinity purified.
• Solubilization
  The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
• Storage condtions
  Keep the TdT Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
• Tips
  The TdT Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
• Background
  Terminal Deoxynucleotidyl Transferase, also known as TDT and terminal transferase, is a unique DNA polymerase without template direction catalyzes the addition of deoxyribonucleotides onto the 3-prime-hydroxyl end of DNA primers. Its gene is mapped to the region 10q23-q24. And TDT cDNA contains an open reading frame of 1,530 basepairs corresponding to a protein containing 510 amino acids. TDT may be responsible for inserting nucleotides (N regions) at the V(H)-D and D-J(H) junctions of immunoglobulin genes. The enzyme is present in immature thymocytes, some bone marrow cells, transformed pre-B and pre-T cell lines, and leukemia cells. Additionally, TDT catalyses the addition of nucleotides to the 3' terminus of a DNA molecule. Unlike most DNA polymerases it does not require a template. The preferred substrate of this enzyme is a 3'-overhang, but it can also add nucleotides to blunt or recessed 3' ends. Cobalt is a necessary cofactor.
• Related articles
  1. Landau, N. R.; St. John, T. P.; Weissman, I. L.; Wolf, S. C.; Silverstone, A. E.; Baltimore, D. : Cloning of terminal transferase cDNA by antibody screening. Proc. Nat. Acad. Sci. 81: 5836-5840, 1984. 2. Yang-Feng, T. L.; Landau, N. R.; Baltimore, D.; Francke, U.: The terminal deoxynucleotidyltransferase gene is located on human chromosome 10 (10q23-q24) and on mouse chromosome 19. Cytogenet. Cell Genet. 43: 121-126, 1986. 3. Riley, L. K.; Morrow, J. K.; Danton, M. J.; Coleman, M. S.: Human terminal deoxyribonucleotidyltransferase: molecular cloning and structural analysis of the gene and 5-prime flanking region. Proc. Nat. Acad. Sci. 85: 2489-2493, 1988.
• Gene Name
  DNTT
• Protein Name
  DNA nucleotidylexotransferase
• Gene Full Name
  DNA nucleotidylexotransferase
• Synonyms
  Deoxynucleotidyltransferase terminal antibody|DNA nucleotidylexotransferase antibody|DNTT antibody|Nucleosidetriphosphate DNA deoxynucleotidylexotransferase antibody|TDT antibody|TDT_HUMAN antibody|Terminal addition enzyme antibody|Terminal deoxynucleotidyltransferase antibody|Terminal deoxyribonucleotidyltransferase antibody|Terminal transferase antibody
• Uniprot ID
  P04053
• Entrez GeneID
  1791
• Properties
  If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  TdT  
• Gene symbol
  DNTT, POLM, DNTTIP1
• Short name
  TdT Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Alternative name
  TdT (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:2983
• Gene
  DNTT
• Long gene name
  DNA nucleotidylexotransferase
• Synonyms gene name
  • deoxynucleotidyltransferase, terminal
• Synonyms
  • TDT
• Synonyms name
  • Terminal deoxynucleotidyltransferase
• GenBank acession
  • AB046378
• Locus
  10q24.1
• Discovery year
  2001-06-22
• Entrez gene record
  1791
• RefSeq identity
  • NM_004088
• Classification
  • DNA polymerases
• VEGA ID
  OTTHUMG00000018832

Gene info

• Identity
  HGNC:9185
• Gene
  POLM
• Long gene name
  DNA polymerase mu
• Synonyms gene name
  • polymerase (DNA directed), mu
  • polymerase (DNA) mu
• Synonyms
  • Tdt-N
• Synonyms name
  • Pol iota
• GenBank acession
  • AF176097
• Locus
  7p13
• Discovery year
  2000-02-16
• Entrez gene record
  27434
• Pubmed identfication
  • 10982892
• RefSeq identity
  • NM_013284
• Classification
  • DNA polymerases
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000155353

Gene info

• Identity
  HGNC:16160
• Gene
  DNTTIP1
• Long gene name
  deoxynucleotidyltransferase terminal interacting protein 1
• Synonyms gene
  • C20orf167
• Synonyms gene name
  • chromosome 20 open reading frame 167
  • deoxynucleotidyltransferase, terminal, interacting protein 1
• Synonyms
  • dJ447F3.4
  • Tdif1
• Synonyms name
  • novel protein similar to synaptotagmin 1 (SYT1, P65) (isoform 1)
  • TdT binding protein
• GenBank acession
  • AB035676
• Locus
  20q13.12
• Discovery year
  2001-07-17
• Entrez gene record
  116092
• Pubmed identfication
  • 11473582
• RefSeq identity
  • NM_052951
• Classification
  • MiDAC complex subunits
• VEGA ID
  OTTHUMG00000032610

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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