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Target antigen
Syndecan 2
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Clonality
Polyclonal antibody
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Clone
Polyclonal antibody
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Raised in
rabbit
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Type of the antibody
IgG polyclonal antibody
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Product form
freeze-dried
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Reacts with species
human, rat Predicted to work with: mouse
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Analyses
WB,IHC-P,IHC-F
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Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Syndecan 2(105-122aa EETDKEKVHLSDSERKMD).
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Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
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Purification
Immunogen affinity purified.
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Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
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Storage condtions
Keep the Syndecan 2 Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The Syndecan 2 Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
SDC2(Syndecan-2) also known as SYND2, HSPG1, HSPG, and Fibroglycan, is a protein that in humans is encoded by the SDC2 gene. The protein encoded by this gene is a transmembrane(type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. By Southern hybridization to a panel of human-mouse somatic cell hybrid DNA and by in situ hybridization, Marynen et al.(1989) showed that the heparan sulfate proteoglycan core protein maps to 8q22-q24.(Heparan sulfate proteoglycan of basement membrane appears to be encoded by a gene on human chromosome 1.Bobardt et al.(2003) demonstrated that syndecans, including SDC2, can function as in trans HIV receptors via binding of HIV-1 gp120 to the syndecan heparan sulfate chains. Flow cytometric analysis demonstrated SDC expression on endothelial cells. HIV bound to SDC on endothelial cell lines maintained its infectivity for at least 1 week, compared with less than 1 day for unbound virus. Bobardt et al.(2003) suggested that SDC-rich endothelial cells lining the vasculature can provide a microenvironment that boosts HIV replication in T cells.
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Related articles
1. Bobardt, M. D., Saphire, A. C. S., Hung, H.-C., Yu, X., Van der Schueren, B., Zhang, Z., David, G., Gallay, P. A.Syndecan captures, protects, and transmits HIV to T lymphocytes.Immunity 18: 27-39, 2003. 2. David, G., van der Schueren, B., Marynen, P., Cassiman, J.-J., van den Berghe, H.Molecular cloning of amphiglycan, a novel integral membrane heparan sulfate proteoglycan expressed by epithelial and fibroblastic cells.J. Cell Biol. 118: 961-969, 1992. 3. Marynen, P., Zhang, J., Cassiman, J.-J., Van den Berghe, H., David, G.Partial primary structure of the 48- and 90-kilodalton core proteins of cell surface-associated heparan sulfate proteoglycans of lung fibroblasts: prediction of an integral membrane domain and evidence for multiple distinct core proteins at the cell surface of human lung fibroblasts.J. Biol. Chem. 264: 7017-7024, 1989.
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Gene Name
SDC2
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Protein Name
Syndecan-2
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Gene Full Name
syndecan 2
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Synonyms
CD362 antibody|Fibroglycan antibody|Heparan sulfate proteoglycan core protein antibody|HSPG antibody|SDC2 antibody|SDC2_HUMAN antibody|SYND2 antibody|Syndecan-2 antibody
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Uniprot ID
P34741
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
