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Target antigen
Securin
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Clonality
Polyclonal antibody
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Clone
Polyclonal antibody
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Raised in
rabbit
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Type of the antibody
IgG polyclonal antibody
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Product form
freeze-dried
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Reacts with species
human, rat Predicted to work with: mouse
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Analyses
WB
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Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Securin(146-161aa LMILDEERELEKLFQL), different from the related rat sequence by four amino acids, and from the related mouse sequence by five amino acids.
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Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
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Purification
Immunogen affinity purified.
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Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
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Storage condtions
Keep the Securin Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The Securin Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
Securin, also known as pituitary tumor-transforming 1 or EAP1 is a protein that in humans is encoded by the PTTG1 gene. PTTG1 gene is mapped to 5q33.3. The encoded protein is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex(APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors. The gene product contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box(D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. The gene product is mainly a cytosolic protein, although it partially localizes in the nucleus.
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Related articles
1. Holt, L. J., Krutchinsky, A. N., Morgan, D. O. Positive feedback sharpens the anaphase switch. Nature 454: 353-357, 2008. 2. Ying, H., Furuya, F., Zhao, L., Araki, O., West, B. L., Hanover, J. A., Willingham, M. C., Cheng, S. Aberrant accumulation of PTTG1 induced by a mutated thyroid hormone beta-receptor inhibits mitotic progression. J. Clin. Invest. 116: 2972-2984, 2006.
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Gene Name
PTTG1
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Protein Name
Securin
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Gene Full Name
pituitary tumor-transforming 1
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Synonyms
EAP1 antibody|PTTG antibody|TUTR1 antibody|Hpttg antibody|TUMOR-TRANSFORMING 1 antibody|Esp1-associated protein antibody|ESP1-ASSOCIATED PROTEIN 1 antibody|Tumor-transforming protein 1 antibody|Pituitary tumor-transforming gene 1 protein antibody
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Uniprot ID
O95997
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
